HPLC同时测定灯盏细辛提取物中3种有效成分的含量

HPLC Determination of Three Active Components in Extract of Erigerontis Herba

目的:建立同时测定灯盏细辛提取物中野黄芩苷,3,5-二-O-咖啡酰奎宁酸和3,4-二-O-咖啡酰奎宁酸的含量测定方法。方法:采用高效液相色谱法,大连依利特C18色谱柱(4.6mm x 250 mm,5μm),流动相乙腈-0.1%磷酸(22∶78),检测波长330 nm,流速1 m L·min-1,柱温35℃。结果:野黄芩苷,3,5-二-O-咖啡酰奎宁酸和3,4-二-O-咖啡酰奎宁酸分别在0.032 7~1.308 0μg(r=0.999 9),0.048 2~1.928 0μg(r=0.999 9),0.058 3~2.332 0μg(r=0.999 8)呈良好的线性关系,加样回收率分别为99.74%(RSD=1.9%),100.23%(RSD=1.4%),99.33%(RSD=1.3%)。结论:本法简便、快捷,结果准确、重复性好,可用于灯盏细辛提取物中3种有效成分的含量测定。

Objective： To establish a method for determination of scutellarin, 3, 5-dicaffcoylquinic acid （3, 5-DCQA）, 3, 4-dicaffeoylquinic acid （3, 4-DCQA） in extract of Erigerontis Herba. Method： The HPLC method was carried out on Dalian Elite C18 column （4.6 mm ×250 mm, 5 μm） evaluated with acetonitrile- 0. 1% phosphoric acid （22：78） as mobile phase, the flow rate was 1 mL.min-1, the temperatue of column was at 35 ℃ , the detection wavelength was at 330 nm for UV detection. Result： The calibration curves were linear in the range of 0.0327 to 1.308 0 μg （r=0.9999） for scutellarin, 0.048 2 to 1.9280 μg （r=0.9999） for 3, 5- DCQA, 0. 058 3 to 2. 332 0 μg （ r = 0. 999 8） for 3, 4-DCQA, respectively. The average recoveries were 99.74% with RSD 1.9% ; 100.23% with RSD 1.4% and 99.33% with RSD 1.3% respectively. Conclusion： The assay demonstrated that the method was simple, it had adequate accuracy and selectivity to quantify the three active components in extract of Erigerontis Herba.