清肺口服液对RSV感染哮喘急性发作小鼠肺组织炎症及气道反应性的影响

Qingfei Oral Liquid Reduced Airway Inflammation and Hypersensitivity in Respiratory Syncytial Virus( RSV) Infected Murine Asthma Model

目的：探讨清肺口服液对呼吸道合胞病毒（RSV）感染哮喘急性发作小鼠模型肺组织炎症及气道反应性的影响。方法：60只雄性6~8周龄Balb/C小鼠,每组10只,随机分为正常组,模型组,清肺口服液高、中、低剂量组（2.34,1.17,0.59 g·kg-1·d-1）,利巴韦林组（0.1 g·kg-1·d-1）,共6组,卵细胞（OVA）致敏及雾化激发哮喘模型,经RSV隔日滴鼻连续3次感染,获得RSV感染哮喘模型;治疗组予清肺口服液分高、中、低剂量ig;正常予等量生理盐水ig：阳性药组给予利巴韦林注射液ip。末次激发24 h后,用Buxco RC系统检测小鼠气道反应性;收集支气管肺泡灌洗液（BALF）进行嗜酸细胞分析;肺组织经HE染色,PAS染色,VG染色评估炎症反应。结果：与正常组比较,模型组小鼠BALF中嗜酸粒细胞比例（EOS%）明显升高,气道反应性较正常组明显增高（P〈0.01）,模型组小鼠肺组织呈现哮喘合并感染典型改变,组织充血水肿,分泌物显著增多;气道周围炎性细胞浸润、胶原增生显著;与模型组比较,清肺口服液中剂量组BALF中EOS%明显降低,清肺口服液及利巴韦林干预均可显著改善气道高反应性（P〈0.05）,其中中剂量清肺口服液效果尤为显著,气道功能与正常组相比差异无显著性,清肺口服液中剂量可以明显减轻小鼠肺组织充血水肿及炎性细胞浸润,气道分泌物及气道周围胶原增生较模型组明显改善。结论：中剂量清肺口服液可以明显减轻RSV感染哮喘急性发作小鼠肺组织炎症及气道高反应性。

Objective： To study the effects of Qingfei oral liquid on airway inflammation and hypersensitivity in a respiratory syncytial virus （RSV） infected murine asthma model. Method： Sixty 6-8-week- old male Balb/C mice were equally divided into six groups by random： the normal group （CON） , model group （ RSV ＋ OVA）, Qingfei oral liquid group [ high dose （ HD）, middle dose （ MD）, low dose （LD） ], and ribavirin group （ LB）. The asthma model was reproduced by sensitization and nebulizer inspired OVA, followed by repeated inhalation of RSV for three consecutive times to obtain RSV-infected asthma model （ 1.0 × 10^6 PFU/mL in 50 μL）. Qingfei oral liquid groups were administered with different doses. The normal control group was administered with normal saline. LB group received ribavirin injection ip 24 h after of the last administering, Buxco RC system was used to detect airway hyperresponsiveness, ESO cells infiltration were measured in bronchoalveolar lavage fluid （BALF） fluid, and inflammation was assessed in lung tissue by HE staining, PAS staining, VG staining. Result： The proportion of BALF＇s eosinophils （ EOS% ） in model group was significantly higher than that in the normal group （P 〈 O. 05）. Airway hyperresponsiveness in model group was significantly higher than that in normal group （P 〈 0.01 ）. Qingfei oral liquid and Ribavirin cleared RSV＇s infection and significantly improved airwayhyperresponsiveness compared with the model group （P 〈 0.05 ） , among which Qingfei oral liquid middle-dose group had the most significant effect. There was no significant difference in airway functions compared with normal group. Qingfei oral liquid middle-dose group significantly reduced lung tissue edema and inflammatory cell infiltration, airway secretions and collagen hyperplasia around airway compared with model group. Conclusion： Qingfei oral liquid middle-dose group can significantly reduce inflammation of lung tissues and airway hyperresponsiveness in RSV infected murine asthma model.