黄皮叶提取物对哮喘大鼠血清及肺组织Th1/Th2平衡的调节作用

Regulating Effects of Clausenae Lansii Folium Extracts on Th1 / Th2 Balance in Serum and Lung Tissue of Asthmatic Rats

目的:探讨黄皮叶提取物对哮喘大鼠炎症反应中Th1/Th2细胞因子平衡的调节作用。方法:将健康雄性SD大鼠48只,随机分为6组,分别为正常组、哮喘模型组、地塞米松组(1.5 mg·kg-1·d-1)、黄皮叶提取物低、中、高剂量组(520,1 040,2 080 mg·kg-1·d-1)。除正常组外,其余各组分别在第1,7天采用卵清蛋白致敏法ih致敏液,并于第15天开始,各给药组大鼠每天在灌胃给药后1 h进行雾化激发,连续1周;正常组及模型组以生理盐水灌胃代替。分别采用ELISA法测定大鼠血清中一氧化氮(NO),白三烯D4(LTD4),白细胞介素-4(IL-4),γ-干扰素(IFN-γ)的含量、硝酸还原法测肺组织NO含量;RT-PCT法测定肺组织中IL-4,IFN-γmRNA表达及观察肺组织病理学改变。结果:与正常组比较,模型组大鼠血清中NO,IL-4,LTD4的含量明显升高,IFN-γ的含量降低,肺组织NO含量明显升高,大鼠肺组织中IL-4 mRNA表达明显升高,IFN-γmRNA表达明显降低(P<0.01);与模型组比较,黄皮叶提取物低、中、高剂量组均能明显减少血清中NO,IL-4的含量,升高IFN-γ的含量(P<0.05,P<0.01),黄皮叶提取物高、中剂量组能减少哮喘大鼠肺组织中NO的含量,黄皮叶提取物高剂量组能明显减少哮喘大鼠血清中LTD4的含量(P<0.05,P<0.01),黄皮叶提取物低、中、高剂量组能明显降低哮喘大鼠肺组织中IL-4mRNA表达,增加IFN-γmRNA表达(P<0.05,P<0.01)。结论:黄皮叶提取物能有效的减少哮喘大鼠的炎症反应,其机制可能是通过调节Th1/Th2细胞因子的平衡,从而减轻炎症细胞浸润有关。

Objective： To explore the regulating effects of Clausenae Lansii Folium extracts on cytoskines level of type Th1/Th2 in inflammation reaction on asthmatic rats. Method： Forty-eight healthy male rats were randomly divided into six groups： normal control group, asthma model group, dexamethasone group （1.5 mg.kg-1.d-1）, Clausenae Lansii Folium extracts groups of low, medium and high doses （520, 1 040, 2 080 mg .kg-1. d-1）. Except normal control group, all the other groups were sensitized with ovalbum by subcutaneous injection on the first and the seventh day. From the fifteenth day, rats in various administering groups received atomization treatment 0.5 hour after gavage 1 hour for 1 week. The normal control group and asthma model group were treated with normal saline instead. The levels of nitric oxide （NO）, leukotriene D4 （LTD4 ）, interleukin （IL-4） , interferon-γ （IFN-γ） in serum were measured by ELISA, and NO in lung tissue was detected by nitrate reductase method. The expression of IL-4, IFN-γ mRNA in lung tissue was measured by RT-PCR. The pathological changes in lung tissue were observed. Result： Compared with the normal group, levels of NO, IL-4 and LTD4 in serum of rats in model group were significantly higher, and IFN-γ content was lower. NO content in lung tissues was also significantly higher, IL-4 mRNA expression was significantly higher, and IFN-γ mRNA wassignificantly lower （P 〈 0.01 ）. Compared with the model group, all of Clausenae Lansii Folium groups of low, medium and high dose could significantly reduce NO and IL-4 levels in serum, and increase IFN-γ level （P 〈 0.05, P 〈0.01）. Clausenae Lansii Folium groups of high, medium doses could reduce the level of NO in lung tissues of the asthma rats. Clausenae Lansii Folium high dose group could significantly reduce the level of LTD4 in serum of asthma rats （P 〈0.05, P 〈0.01 ）. All of Clausenae Lansii Folium groups of low, medium and high dose could significantly reduce IL-4 mRNA expression and increase IFN-γ mRNA expression （P 〈 0.05, P 〈 0.01 ）. Conclusion： The extracts of Clausenae Lansii Folium are effective in treatment of inflammation reaction in asthmatic rats by adjusting Th1/Th2 balance, and reducing the infiltration of inflammation cells.