芪七连胶囊对自发性高血压大鼠左心室肥厚的保护作用

Protective Effect of Qiqilian Capsule Against Left Ventricular Hypertrophy of Spontaneously Hypertensive Rats

目的:观察芪七连胶囊对自发性高血压大鼠(spontaneously hypertensive rats,SHR)左心室肥厚的保护作用,并探讨其可能的作用机制。方法:取12周龄的SHR,随机分为模型组,氯沙坦组(4 mg·kg-1),芪七连胶囊组高、中、低剂量组(1.2,0.6,0.3 g·kg-1),每组10只,另取10只健康WKY大鼠作为正常组,正常组ig给生理盐水,给药组ig给相应药物,连续给药6周。每周无创尾套法测量大鼠尾动脉收缩压,采用ELISA测定血清中肿瘤坏死因子-α(tumor necrosis factor alpha,TNF-α),白介素-1β(interleukin-1β,IL-1β),白介素-10(interleukin-10,IL-10)及高敏C-反应蛋白(high-sensitivity C-reactive protein,hs-CRP)的含量,观察各组大鼠心肌肥厚参数及心肌形态学变化。结果:与正常组比较,模型组SHR血压明显升高,血清中TNF-α,IL-1β,hs-CRP含量明显升高,IL-10的含量明显降低(P<0.01),左心室肥厚指数(LVHI),左心室湿质量(left ventricular weight,LVW)/右心室湿质量(right ventricular weight,RVW),心肌纤维直径(myocardial fiber diameter,MD)明显增加(P<0.01);与模型组比较,芪七连胶囊高、中、低剂量组能明显降低SHR血压,明显降低血清中TNF-α,IL-1β,hs-CRP含量,增加血清中IL-10的含量(P<0.01),并能明显降低SHR大鼠的LVHI,LVW/RVW,MD的水平(P<0.05,P<0.01)。病理学检测发现,模型组可见心肌细胞弥漫性肥大,畸形、核大、深染,心肌纤维轻度水肿,排列紊乱,炎性细胞浸润明显,各给药组均能明显改善。结论:芪七连胶囊对SHR左心室肥厚具有保护作用,其作用机制可能与抑制炎性因子的表达有关。

Objective： To observe the protective effect of Qiqilian capsule on the left ventricular hypertrophy （LVH） of spontaneously hypertensive rats （SHR） and explore the possible mechanisms. Method： Twelve-week-old SHRs were divided into the model group, the losartan group （4 mg .kg-1） and Qiqilian capsule high, middle and low dose groups （ 1.2, 0.6, 0.3 g .kg-1 ） , with 10 rats in each group. Another 10 healthy WKY rats were included in the normal group and given normal saline, ig. All of treatment groups were given corresponding drugs, ig, for consecutively six weeks. The blood pressure were measured by tail-cuff, and the method of ELISA was used to determine the contents of serum tumor necrosis factor alpha （TNF-α） , interleukin-1β （IL-1β）, interleukin-10 （IL-10） and high-sensitivity C-reactive protein （hs-CRP）. Changes of cardiac hypertrophy parameters of SHRs were observed. Result： Compared with the normal group, the model group showed significant higher SHR blood pressure, serum TNF-α, IL-1β, hs-CRP, left ventricular hypertrophy index （LVH） , left ventricular weight （LVW） , right ventricular weight （RVW） , myocardial fiber diameter （MD） andlower IL-10 content （P 〈 0.01 ）. The Qiqilian capsule high, middle-and-low-dose groups showed significantly lower blood pressure of SHRs, TNF-α, IL-1β, hs-CRP, lower IL-10 content （P 〈 0.01） and lower LVHI, LVW/ RVW, MD in SHR rats （P 〈 0. 05, P 〈 0.01 ）. According to the pathological detection, the model group showed diffuse enlargement, deformity, large nucleus, hyperchromic effect in myocardial cells, mild edema and disorder arrangement in myocardial fibers arranged, and significant inflammatory cell infiltration; all treatment groups showed obvious mitigations. Conclusion： Qiqilian capsule has the protective effect against LVH in SHRs, and the mechanism may be related to inhibition of the expression of inflammatory cytokines.