陈元膏膏摩对膝骨性关节炎大鼠血清NO和PGE_2及下肢功能的影响

Effect of Chenyuan Ointment Rubbing on Serum NO and PGE_2 and Lower Limb Function of Rats with Knee Osteoarthritis

目的:观察陈元膏膏摩对膝骨性关节炎大鼠血清中一氧化氮(NO)和前列腺素E2(PGE2)及下肢功能恢复的影响,探讨陈元膏膏摩对膝骨性关节炎是否具有抗炎作用。方法:将52只SD大鼠随机分为正常组、模型组、陈元膏膏摩组(2 g/只)、和手法对照组(凡士林,2 g/只),每组13只,除正常组外,其余各组采用石膏固定法建立膝骨性关节炎动物模型,8周后,正常组、模型组不作干预,对陈元膏膏摩组、手法对照组大鼠分别给予陈元膏结合手法和凡士林结合手法治疗,每日1次,持续8周后,HE染色对各组关节软骨病理改变进行Mankin评分,并检测各组血清中NO和PGE2的浓度水平。结果:与正常组比较,模型组软骨病理改变进行Mankin评分和血清NO,PGE2的浓度水平明显升高(P<0.01),其大腿周长和膝关节活动度均有明显降低(P<0.01);陈元膏膏摩组、手法对照组Mankin评分和血清NO,PGE2的浓度水平均低于模型组(P<0.01),其中陈元膏膏摩组的血清NO,PGE2浓度水平较低(P<0.05),陈元膏膏摩组、手法对照组的大鼠在治疗后,其大腿周长和膝关节活动度均有明显提高(P<0.01),其中陈元膏膏摩组提高的幅度较大(P<0.05)。结论:陈元膏膏摩疗法可降低KOA大鼠血清中NO和PGE2的浓度水平,更好地促进大鼠大腿肌力和膝关节活动度的改善,对减轻膝骨性关节炎症状具有一定的作用。

Objective： To observe the effect of Chenyuan ointment rubbing on the serum levels of nitric oxide （NO） and prostaglandin E2 （PGE2 ） of rats with knee osteoarthritis （KOA） and explore its anti- inflammatory effect. Method： Totally 52 SD rats were randomly divided into 4 groups ： normal group （ group A） , model group （group B） , Chenyuan ointment group （group C, 2 g/rat） and manipulation control group （group D, Vaseline, 2 g/rat） , with 13 rats in each group. KOA model was induced by closed-plaster method, except for the normal group. Eight weeks after fixation, groups A and B were given no intervention, the rats in groups C and D were given Chenyuan ointment with manipulation and vaseline with manipulation once a day. After 8 weeks, HE staining was adopted to observe the pathological changes of articular cartilage in each group and Mankin score and detect the serum levels of NO and PGE2. Result： Compared with group A, group B showed significantly higher mankin score and serum NO and PGE2 （P 〈 0.01 ） and lower lower limb circumference and knee joint activity （P 〈0.01 ）. Groups C and D showed lower mankin score and the serum levels of NO and PGE2 than that of group B （P 〈 0.01 ）. Specifically, group C showed the lower serum levels of NO and PGE2 （P 〈 0.05）. After thetreatment, limb circumference and activity of knee joint of rats were significantly improved in groups C and D （P 〈 0.01） , particularly group C were improved more greatly （P 〈 0.05）. Conclusion： Chenyuan ointment rubbing can reduce the concentration level of NO and PGE2 in serum of KOA rats, and better improve muscle strength of lower limbs and activity of knee joint. Moreover, it has a ertain effect in reducing joint inflammation.