昌都锦鸡儿异黄酮类成分及其抑制NO生成活性

Isoflavonoids from Caragana changduensis and their nitric oxideinhibitory activities

采用多种柱色谱分离技术从昌都锦鸡儿根茎木部心材的95%乙醇提取物中分离得到10个异黄酮类化合物。通过波谱数据分析及与文献对照鉴定其结构分别为7,2＇-二羟基-8,4＇-二甲氧基异黄酮（1）,4＇-羟基-7,3＇-二甲氧基异黄酮（2）,5,7,4＇-三羟基-2＇,5＇-二甲氧基异黄酮（3）,樱黄素（4）,阿夫罗摩辛（5）,奥刀拉亭（6）,染料木素（7）,蓝花赝靛素（8）,红车轴草素（9）,6,7,3＇-三羟基-4＇-甲氧基异黄酮（10）。其中,化合物1~3,9~10为首次从该属植物中分离得到。所有化合物均为首次从该植物中分离得到。体外活性测试结果显示化合物1,2,6,7具有一定的抑制大鼠巨噬细胞NO生成活性,其IC50分别为48.12,25.32,62.71,43.59μmol·L-1。

Ten isoflavonoids were isolated from the heartwoods of Caragana changduensis Lion f. by means of various column chro- matographic techniques. Based on the detailed spectral data analysis （ MS and NMR） , as well as comparison with the literatures, their chemical structures were determined as 7,2＇-dihydroxy-8,4＇-dimethoxyisoflavone （ 1 ） , 4＇-hydroxy-7,3＇-dimethoxyisoflavone （2）, 5,7, 4＇-trihydroxy-2＇,5＇-dimethoxyisoflavone（3）, prunetin（4）, afrormosin（5）, odoratin（6）, genistein（7）, texasin（8）, pratensein（9）, and 6,7,3＇-trihydroxy-4＇-methoxyisoflavone（10）. Among them, compounds 1-3 and 9-10 were isolated from the Caragana genus for the first time. All the compounds were obtained from this species for the first time. In the preliminary assays, compounds 1, 2, 6, and 7 pos- sessed significant inhibitory effects on NO production, with IC50 values of 48. 12, 25. 32, 62. 71,43.59 μmol · L-1, respectively.