固定化Cu^(2+)磁性壳聚糖微球的制备及应用研究

Preparation and application of magnetic chitosan microspheres immobilized Cu^(2+)

采用化学共沉淀法合成Fe3O4纳米颗粒,乳化交联法制备磁性壳聚糖微球。以环氧氯丙烷为活化剂,螯合铜离子制备了固定化Cu2+的磁性壳聚糖微球(Cu2+-IDA-MCS)为亲和介质。以乳源蛋白中发现的血管紧张素转化酶抑制肽(ACEI)Val-Ser-Leu-Pro-Glu-Try(VSLPEW)为模型分子,考察了Cu2+-IDA-MCS对VSLPEW的吸附效果。结果表明,固定化Cu2+的磁性壳聚糖微球吸附VSLPEW的最佳条件为:VSLPEW的初始浓度为2.0mg/mL,亲和介质的吸附时间为40min,吸附温度为30℃,缓冲液pH值为7.5,此时Cu2+-IDA-MCS对VSLPEW的最大吸附量达49.08mg/g。说明Cu2+-IDA-MCS是吸附VSLPEW的一种有效的亲和介质。

Fe3O4 used in the preparation of the magnetic chitosan microspheres were prepared by chemical coprecipitation. Magnetic chitosan microspheres were prepared by the emulsion crosslinking technique. Then the material was activated with epichlorohydrin,chelated by the Cu^2＋. Finally, the ACEI（Val-Ser-Leu-Pro-Glu-Try） was used as a model to be researched under different concentrations. The results showed that the optimal conditions for adsorbing were immobilization initial concentration 2.0 mg/mL, time 40 min, temperature 30 ℃, pH ： 7.5, and the adsorption capacity of the affinity media for ACEI can reach 49.08mg·g^-1. The magnetic chitosan microspheres immobilized Cu^2＋ can be used as an effective affinity media to adsorb ACEI.