调控雪旺细胞迁移能力相关分子通路的初步研究

A pilot study of the regulatory signaling pathway related to Schwann cell migration

目的初步研究调控雪旺细胞迁移的信号传导通路。方法细胞划痕实验对比静息态与激活态雪旺细胞迁移能力，用Westernblot方法测定各自ERKl／2和AKT蛋白表达。应用相应蛋白通路抑制剂后观察雪旺细胞迁移能力改变及ERKl／2和AKT蛋白表达，初步探索雪旺细胞迁移信号通路。结果激活态雪旺细胞迁移能力较静息雪旺细胞明显提高，同时伴随着p-ERK表达增加。单纯阻断ERK磷酸化对激活态雪旺细胞迁移的能力无明显影响，而且伴随着p-AKT表达增加。联合阻断ERK及AKT磷酸化可明显降低激活态雪旺细胞的迁移能力。结论雪旺细胞的迁移能力与ERK及AKT通路联合调控相关。

Objective To explore the cell signaling pathway involved in Schwann cell （SCs） migration. Methods Quiescent Schwann cells （qSCs） and activated Schwann cells （aSCs） were cultured and then identified by imunnostaining of S100 and p75NGFR. Cell mobility was determined by the wound healing assay. Western blot was adopted to detect ERK1/2, ATK and their phosphorylation style. SC migration signaling pathway was explored by observing changes in cell mobility and ERK1/2 and AKT expression upon inhibition of the pathways. Results The mobility of aSCs was higher than that of qSCs. Elevated p-ERK1/2 was coupled with the migration of SCs, activated by nerve degeneration. Simple inhibition of ERK phosphorylation did not show significant negative effect on the migratory potential of aSCs, but elevation of p-AKT expression. Combined inhibition of ERK and AKT phosphorylation resulted in a significant decrease in aSCs mobility. Conclusion Both ERK and AKT pathways are involved in Schwann cell migration.