复方龙脉宁提取液对小鼠急性心肌缺血缺氧损伤的保护作用

Protective Effect of Compound Longmaining Extract on Acute Myocardial Ischemia Hypoxia Injury in Rats

目的研究复方龙脉宁水提液和醇提液对小鼠耐缺氧以及对小鼠急性心肌缺血损伤的保护作用。方法取昆明种小鼠随机分为9组,每组8只,分别为正常组,模型组,地奥心血康组(0.166 g·kg-1),复方龙脉宁水提液高、中、低剂量组(24,16,8 g·kg-1),复方龙脉宁醇提液高、中、低剂量组(24,16,8 g·kg-1),连续灌胃给药7 d,每天1次,正常组与模型组给予等量生理盐水。除正常组外,其余各组于末次灌胃30 min后,皮下注射异丙肾上腺素(ISO,20 mg·kg-1)诱发小鼠心肌缺氧实验以及建立小鼠急性心肌缺血模型,观察复方龙脉宁水提液和醇提液对小鼠密闭缺氧存活时间的影响,以及对心脏质量指数、乳酸脱氢酶(LDH)活力、肌酸激酶(CK)活力、丙二醛(MDA)含量以及心肌组织病理形态学的影响。结果复方龙脉宁水提液和醇提液的高、中剂量组小鼠存活时间均不同程度高于模型组(P<0.05,P<0.01);与模型组比较,复方龙脉宁水提液和醇提液中剂量组可降低血清LDH、CK活力(P<0.05,P<0.01),减少MDA含量(P<0.05),复方龙脉宁醇提液的高剂量组可降低小鼠缺血心肌的心脏质量指数(P<0.05),改善缺血性心肌肥厚;复方龙脉宁两种提取液的高、中剂量组均对心肌组织病理损伤有不同程度的改善作用。结论复方龙脉宁不同提取液的适宜剂量能提高小鼠耐缺氧能力以及对小鼠急性心肌缺血损伤具有明显的保护作用,其作用机制可能与减少心肌耗氧、保护心肌细胞膜、抗氧自由基等有关。

Objective To observe the protective effect of water extract and alcohol extract of compound Longmaining （CL） on hypoxia tolerance and acute myocardial ischemia injury in rats. Methods Seventy-two Kunming mice were randomized into 9 groups, 8 mice in each group. The 9 groups were normal group, model group, Di＇ao Xinxuekang group（0.166 g·kg-l）, high-, middle- and low-dose CL water extract groups（24, 16, 8 g·kg-1）, and high-, middle- and low-dose CL alcohol extract groups（24, 16, 8 g· kg-1）. The mice were medicated by gastric gavage, and the normal group and model group were given the same volume of saline, once a day for 7 days. Except for the normal group, mice of the other groups were given subcutaneous injection of isoproterenol （20 mg· kg-1） 30 min after the last medication to induce myocardial hypoxia and acute myocardial ischemia. And then we observed the effects of CL water extract and CL alcohol extract on the survival time of rats under hypoxia condition, and their effects on heart mass index （ HMI ）, malondialdehyde （ MDA ） content, lactate dehydrogenase （ LDH ） activity, creatine kinase（ CK）activity and myocardial tissue pathologic morphology. Results Compared with the model group, high- and middle-dose CL water extract and alcohol extract（24, 16g·kg-1） could prolong the mice survival time to various degrees（P 〈 0.05, P〈 0.01） reduce HMI（P 〈 0.05）, relieve ischemic myocardial hypertrophy, and improve the myocardial pathological changes to various degrees. Middle-dose CL water extract and alcohol extract could decrease LDH and CK activity, and reduce MDA content（P 〈 0.05）. Conclusion CL extract in the suitable dose shows remarkable protective effect on myocardial hypoxia in mice and on acute myocardial ischemia injury of mice, and the mechanism may be related to reducing myocardial oxygen consumption, protecting the myocardial cell membrane, and counteracting oxygen free radicals.