HPLC法同时测定猴耳环药材中3种黄酮类成分的含量

Simultaneous Determination of Three Flavonoids in Pithecellobium clypearia by HPLC

目的 建立同时测定猴耳环药材中杨梅苷（1）、5,3’,4’,5’-四羟基黄烷-7-没食子酸酯（2）、槲皮苷（3）含量的方法,并对不同批次猴耳环药材中3种黄酮的含量进行测定。方法 采用Hypersil BDS C18色谱柱（150 mm×4.0 mm,5μm）;流动相：0.2%磷酸-乙腈,梯度洗脱;检测波长：320 nm。结果 3种黄酮分离度良好;各成分质量浓度与峰面积在测定范围内均呈良好的线性关系（r≥0.9994）;平均加样回收率在99.91%∽100.98%之间,RSD为0.36%∽1.20%（n=6）;所测11批猴耳环药材中3种黄酮（1∽3）含量分别在0.36∽2.23 mg·g-1（1）,6.46∽22.41 mg·g-1（2）,1.06∽4.59 mg·g-1（3）之间。结论 该方法简单、准确,具有良好的重复性和稳定性,可作为猴耳环药材的质量控制方法。

Objective To establish a HPLC method for the simultaneous determination of myricintrin （1）, 5, 3＇, 4＇, 5＇-tetrahydroxyilavan-7-gallate（2）, and quercitrin （3） in Pithecellobium clypearia by HPLC, and to determine their contents in different batches of Pithecellobium clypearia. Methods HPLC was performed on Hypersil BDS Cls colum（ 150 mm×4.0 mm, 5 μm） with a mobile phase of acetonitrile and 0.2 % phosphoric acid at a flow rate of 1.0 mL ＂min-1 by gradient elution. The detection wavelength was set at 320 nm. Results The three kinds of flavonoids were separated well. Good linear relationship showed between the concentrations and the peak areas of the three flavonoids （ 1-3 ） in the range of 0.020-0.600μg （r=0.9997）, 0.067-1.995 μg （r=0.9994）, 0.044-1.266μg （r= 0.9994）, respectively. The recoveries of the three flavonoids were between 99.91%-100.98 % , and the relative standard deviations（RSDs） were between 0.36 %-1.20 %. The contents of the three flavonoids in Pithecellobium clypearia from different batches were between 0.36-2.23 mg·g-1 （1） , 6.46-22.41 mg·g-1 （2）, and 1.06-4.59 mg·g-1 （3）. Conclusion The established method is rapid, accurate with high repeatability, and could be used for the quality control of Pithecellobium clypearia from different habitats.