蒙汉族儿童过敏性紫癜MIF-173位点基因多态性的分析比较

Comparison of polymorphism of MIF-173 gene locus with Henoch-Scholein purpura of children between the Han nationality and Moggol nationality

目的通过对内蒙古地区蒙汉族儿童血清中巨噬细胞移动抑制因子（macrophage migratin inhibitory factor，MIF）．173位点基因多态性（分别是CC、CG、GO）的检测，探讨MIF-173基因位点是否与蒙汉族过敏性紫癜（Henoch—Schonlein purpura，HSP）具有相关性，且MIF-173位点基因多态性在蒙汉族HSP之间有无差异。方法选自内蒙古医科大学附属医院和国际蒙医院儿科住院治疗HSP及健康体检的蒙汉族儿童105例，分为4组：即蒙古族HSP组（25例）、汉族HSP组（25例）、蒙古族正常对照组（25例）和汉族正常对照组。各组年龄1～14岁。抽取静脉血2ml作为样本，采用聚合酶链反应和限制性内切酶片段长度多态性（PCR-RFLP）方法检测各组人群基因中MIF-173位点多态性，运用SPSS17．0软件进行统计学处理。结果MIF-173位点可检测出3种基因型：即CC型、CG型和GG型。汉族HSP组与汉族正常对照组相比，MIF-173位点中以CC基因型居多；两组中各基因型相互比较，χ^2=10．46，P〈0．05，差异有统计学意义。蒙古族HSP组与蒙古族正常对照组相比，MIF-173位点中仍以CC基因型居多；两组中各基因型相比,X2=6．61，P〈0．04，差异有统计学意义。而蒙古族HSP组与汉族HSP组MIF-173位点各基因型相比，χ^2=0．099，P=0．952，差异无统计学意义。结论MIF一173基因位点可能参与了HSP的发生和发展；MIF-173位点基因多态性在蒙古族HSP与汉族HSP之间并无差异，说明MIF-173位点基因多态性可能并不是导致蒙古族HSP与汉族HSP病史不同的原因。

Objective To study gene polymorphisms （CC, CG, GG） of macrophage migration in- hibitory factor （MIF） in children of the Han nationality and Moggol nationality and to find that whether MIF- 173 gene position is associated with HSP of the Han nationality and Moggol nationality, and differences between the Han nationality and Moggol nationality in MIF-173 gene position. Methods All cases were enrolled in Inner Mongolia Medical University Affiliated Hospital and Inner Mongolia International Mongolian hospital from December 2012 to December 2013. There are 105 cases of the Han nationality and Moggol nationality with HSP and control groups, Aged from 1 to 14 years old. There are four groups： Moggol nationality with HSP group （ 25 cases） and the group of the Han nationality with HSP （25 cases）, Moggol nationality control group （25 cases） and the Han nationality control group （25 cases）. Each sample was ＆awed from children＇s venous blood 2ml for inspection. We used DNA extraction kit to extract all DNA and used the method of polymerase chain reaction-restriction fragment length polymorphism （PCR-RFLP） to detect the polymorphism of MIF -173 gene loci. SPSS17. 0 statistical software was used for analysis. Results MIF-173 gene position could be detected out three genotypes, GG, CG and CC type. Compared to the group of the Han nationality with HSP and the Han nationality control group, there is mostly MIF-173 CC gene type. the difference is significant statisti- cally in these two groups （χ^2= 10. 46, P 〈0. 05）. Compared to Moggol nationality with HSP group and Mog-gol nationality control group, there was mostly MIF-173 CC gene type. The difference is significant （χ^2 = 6. 61, P 〈 0. 05 ）. Conclusion MIF may play an important role in the occurrence and development of HSP. There were no significant differences between Moggol nationality with HSP group and the Han nationality with HSP group （χ^2=2.299, P〉0.05）.