唾液乳杆菌对哮喘Ba1b／c小鼠血清及肺组织匀浆中IL-4、IFN-γ及IgE的影响

Effect of iactobacillus salivarius on asthma Ba1b/c mouse in IL-4 ,IFN-γ and IgE of serum and lung tissue homogenates

目的探讨唾液乳杆菌对哮喘Ba1b／c小鼠血清及肺组织匀浆中IgE、IL-4及IFN-γ的影响。方法选择30只4W、体重16—18g、SPF级Ba1b／c雌性小鼠，随机分成3组：正常对照组（N）、哮喘组（A）、哮喘＋唾液乳杆菌组（AH）。应用卵蛋白激发方法建立急性哮喘模型，观察小鼠行为学改变、病理HE染色观察气道炎症改变、电镜观察气管纤毛数量变化，ELISA法测定血清及肺组织匀浆中IgE、IL-4、IFN-γ水平。结果A组小鼠在4％卵蛋白生理盐水溶液雾化激发5—10min后出现明显的烦躁不安、头面部瘙痒、抓耳挠腮、呼吸急促、打喷嚏、弓背、前肢缩抬、二便失禁等哮喘急性相表现，AH组小鼠以上反应减轻；病理HE染色见A组支气管管壁增厚、管腔狭窄、气管及血管周围可见大量以嗜酸性粒细胞为主的炎症细胞浸润，管腔中较多炎性分泌物，AH组小鼠肺组织病理改变较哮喘组明显减轻；电镜下，气管纤毛数量A组较N组显著减少，AH组较哮喘组显著增加；血清及肺组织匀浆中A组IgE、IL4表达水平明显高于N组（P〈0．05），IFN-γ表达水平明显低于N组（P〈0．05），而AH组IgE、IL-4表达水平均较A组明显降低，IFN-γ表达水平较A组升高（P〈0．05）。结论致敏前唾液乳杆菌灌胃一定程度上减轻了哮喘小鼠的临床症状，部分纠正了Th1／Th2失衡，降低了哮喘模型小鼠的气道炎症。

Objective To invstigate the effect of Lactobacillus salivarius on asthma Balb/c mouse in IgE,IL-4 and IFN-γ of serum and lung tissue homogenates. Methods Thirty female Balb/c mice weighing 16- 18 g （4 weeks old）, SPF grade, were randomly divided into three groups：normal control group （N）, asthma group（A）, asthma combined LactobaciUus salivarius group （AH）. Acute asthma model was established by ovalbumin challenge method, mice were observed in behavioral changes, non-invasive method for the determina- tion of airway responsiveness in mice, the changes in airway inflammation and the number changes of tracheal cilia were observed by pathological HE staining and the electron microscope. Respectively,the levels of IgE and IL-4/IFN-γ in the serum and lung tissue homogenate were measured by ELISA. Results five - ten rain after 4% OVA saline solution atomization excitation mice in A group appeared obvious restlessness, head and facial itching, scratching, shortness of breath, perioral cyanosis with abdominal breathing, sneezing, stooping, forelimbs shrinkage, urine and feces incontinence and other asthma acute phase performances, The above reaction alleviated in AH group compared to A group;Pathology of HE staining in asthma group showed the thickening bronchial wall, narrow lumen, peribronchial and perivascular infiltration with a large number of eosinophil-based inflamma- tory cells,lumen with many inflammatory secretions, Pathological changes in lung tissue of mice in AH group significantly alleviated compared with asthma group;Under the electron microscope, the number of cilia in A group significantly decreased compared with N group, while AH group significantly increased compared with A group; Expression level of IgE, IL-4 in the serum and lung homogenate of A group was significantly higher than that of N group （ P 〈 0.05 ）, the level of IFN-γ expression was significantly lower than N group （ P 〈 0. 05 ）, however the expression level of IgE, IL-4 in AH group significantly decreased compared with A group （ P 〈 0. 05） ,and IFN-γ expression level elevated compared with A group（P 〈0.05）. Conclusion Lavage with Lac- tobacillus salivarius before sensitization could alleviate clinical symptoms of asthmatic mice, partially correct Th1/Th2 imbalance as well as reduce airway inflammation.