吉林人参低聚肽的免疫调节作用

Immunomodulatory effects of Panax ginseng oligopeptide of Jilin Province

为研究吉林人参低聚肽(GOP)对小鼠的免疫调节作用,选取280只SPF级雌性BALB/c小鼠,随机分为7组:空白对照组、乳清蛋白组(150 mg/kg)及5个GOP组(37.5、75、150、300、600 mg/kg)。连续灌胃30 d后,进行免疫7项实验测定,观察GOP对小鼠免疫器官相对重量、细胞免疫功能、体液免疫功能、单核-巨噬细胞功能和NK细胞活性的影响。结果表明:GOP显著提高了Con A诱导的小鼠脾淋巴细胞增殖能力、迟发型变态反应能力、抗体生成细胞数、小鼠碳廓清指数、巨噬细胞吞噬率和吞噬指数、NK细胞活性(P<0.05),且效果优于乳清蛋白。由此可知,GOP可以通过增强细胞免疫功能、体液免疫功能、单核-巨噬细胞吞噬能力和NK细胞活性,起到增强免疫力的作用。

This paper investigates the immunomodulatory effects of Panax ginseng oligopeptide of Jilin providence. A total of 280 specific pathogen free （SPF） female BALB/c mice are randomly divided into 7 groups： the control group, the whey protein group （whey protein of 150 mg/kg） and five groups of Panax ginseng oligopeptide （GOP） at the doses of 37.5, 75, 150, 300 and 600 mg/kg. After 30 days, seven assays are performed to determine the cell-mediated immunity, the humoral immunity, the macrophage phagocytosis and the natural killer （NK） cell activity in the mice, including the splenic lymphocyte proliferation and the delayed-type hypersensitivity assays （for cell-mediated immunity）, the IgM antibody response of spleen to SRBC and serum hemolysin level assays （for humoral immunity）, the carbon clearance assay and the phagocytic capacity of peritoneal cavity phagocytes assays （for macrophage phagocytosis） and the NK cell activity assays. The results show that the GOP helps significantly to increase the proliferation response of the splenic lymphocyte to the ConA, the delayed-type hypersensitivity to the SRBC, the IgM antibody response of the spleen to the SRBC, the serum hemolysin level, the 37.5 mg/kg macrophage phagocytosis carbon clearance assay, the phagocytic rate （mg/kg） and the phagocytic index, and the NK cell activity （37.5 mg/kg）, and the effects are better than those of the whey protein. In conclusion, the GOP could improve the immune functions in mice, by enhancing the functions of the cell-mediated immunity, the humoral immunity, the macrophage phagocytosis and the NK cell activity.