全长型脾酪氨酸激酶在口腔鳞状细胞癌组织中的表达及其与肿瘤侵袭和转移的关系

Expression of full-length spleen tyrosine kinase in the oral squamous cell carcinoma and its relationship with tumor invasion and metastasis

目的通过检测口腔鳞状细胞癌(OSCC)组织中全长型脾酪氨酸激酶[SYK(L)]的表达、与淋巴结转移的相关性分析,探讨SYK(L)对OSCC发生发展和转移的影响。方法采用实时荧光定量聚合酶链反应(RT-qPCR)、免疫印迹(Western blot)、免疫组织化学法检测SYK(L)在27例OSCC及其癌旁组织中表达的差异,并采用14例正常口腔黏膜作为对照,探讨其与OSCC发生及转移的关系。结果 OSCC组织中SYK(L)表达量明显低于正常牙龈组织(P<0.01),同一患者癌组织中SYK(L)表达量比癌旁组织低(P<0.05);淋巴结转移组患者的SYK(L)表达量普遍低于未转移组患者(P<0.05)。结论 SYK(L)可能与OSCC的发生和发展有关,作为抑癌基因在肿瘤的淋巴结转移过程中起至关重要的作用。

Objective To investigate the expression of full-length spleen tyrosine kinase [SYK （L）] mRNA and protein in human oral squamous cell carcinoma （OSCC） as well as its possible effects on the invasion and metastasis of OSCC. Methods The expression of SYK （L） was detected in 27 cases of OSCC tissues and its matched adjacent non-cancerous tissues by real- time quantitative polymerase chain reaction （RT-qPCR）, Western blot, and immunohistochemistry. Fourteen cases of normal oral gingival tissues were also analyzed as a normal control. Results Reduced mRNA and protein expression of SYK （L） in OSCC tissues was observed compared with that in normal oral gingival tissues （P〈0.01） and adjacent non-cancerous tissues （P〈0.05）. SYK（L） expression was significantly associated with lymph-node metastasis （P〈0.05）. Conclusion SYK（L） is a candidate tumor suppressor for OSCC tissues, and has an inhibitive effect on the initiation, proliferation, and lymph-node metastasis of human OSCC.