摘要
以脐橙果皮为研究对象,比较了室温和热辅助表面解吸常压化学电离源(DAPCI)的解吸电离特性,结合串联质谱技术,对果皮中香气、糖、黄酮类等主要成分进行了鉴定,并通过主成分分析(PCA)方法对热辅助DAPCI-MS所获外果皮指纹谱图信息进行分析,进而区分了3个不同产地脐橙。结果表明,室温DAPCI-MS对脐橙果皮中的香气成分具有较强的解吸电离能力,而热辅助DAPCI-MS则更适合于分析果皮中的黄酮类和糖类及其衍生物。DAPCI-MS具有灵敏度高、分析速度快等特点,有望成为农产品品质快速检测与产地识别研究的新方法。
Comparison between ambient temperature and thermal surface desorption atmospheric pressure chemical ionization mass spectrometry (DAPCI-MS) was made with pericarp of navel orange. The main components including aroma compounds, flavanones and sugars were identified by tandem mass spectrometry. Following the method of thermal DAPCI-MS, the mass spectra of epicarp were obtained and were further analyzed by principle component analysis ( PCA), which allows a confident discrimination of navel orange samples from three different origins. The results show that aroma compounds are desorbed effectively by ambient temperature DAPCI-MS, while flavanones, sugars and its derivatives are analyzed easily by thermal DAPCI-MS. DAPCI-MS is attractive by showing the advantage of high sensitivity and analytical speed, and thus provides a possible new method to rapidly screen the quality of agricultural products and distinguish origins.
出处
《应用化学》
CAS
CSCD
北大核心
2015年第10期1201-1207,共7页
Chinese Journal of Applied Chemistry
基金
国家自然科学基金(21365002
21465002)
长江学者和创新团队发展计划项目(IRT13054)
教育部新世纪优秀人才支持计划(NCET-11-0999)
江西省高等学校科技落地计划项目(KJLD13051)
江西省科技支撑计划(20144BBB70008)资助~~
关键词
表面解吸常压化学电离源
质谱
主成分分析方法
脐橙
surface desorption atmospheric pressure chemical ionization
mass spectrometry
principalcomponent analysis
navel orange