加味甘麦大枣颗粒的定性定量标准研究

Quality Standard Research of Jiawei Ganmai Dazao Granule

目的：研究加味甘麦大枣颗粒的质量标准研究。方法：运用TLC对加味甘麦大枣颗粒中的合欢皮、地龙和甘草进行定性鉴别；采用HPLC对甘草苷和甘草酸铵进行定量测定。结果：各薄层定性色谱中，供试品在与对照药材相应位置血显相同颜色的斑点；甘草鲁在0．035～0．700μg范围内（R12=0．9996），甘草酸铵在0．324—6．488μg范围内（R22=1．0000）与色谱峰峰面积呈良好的线性关系。结论：所用方法简便、重现性好、专属性强，为加味甘麦颗粒质量评价提供了依据。

Objective： To study the quality standard of Jiawei Ganmai Dazao Granule. Methods： By using HPLC, the quantitative analysis on glycyrrhizin was carried out. By using TLC, the qualitative analysis on Cortex Albizziae, Pberetima and Glycyrrhiza was carried out. Results： In TLC, the test samples and the reference drugs in the appropriate location were on the same color spots. Glycyrrhizinate acid showed excellent regression （R1 ^2 = 0.999 6） at 0. 035 - 0. 700 μg; ammonium glycyrrhizinate showed excellent regression （ R22 = 1. 000 0） at 0. 324- 6. 488 μg. Conclusion ： The method is simple and reproducible with strong specificity to provide a basis for quality evaluation of Jiawei Ganmai Dazao Granule.