摘要
目的:建立降脂合剂中5-羟甲基糠醛(5-HMF)、苍术素(atractydin)、丹酚酸B(salvianolic acid B)的高效液相含量测定方法。方法:采用Hibar ODS柱(4.6mm×250mm,5μm),以乙腈-0.1%甲酸溶液为流动相梯度洗脱,流速为1.0mL·min^-1,检测波长为300nm,柱温为35℃,进样量10μL。结果:5-羟基甲基糠醛在7.812~250.0μg·mL^-1范围内线性关系良好(R。=0.9993),回收率为100.03%(RSD=1.76%,n=5),苍术素在7.812—250.0μg·mL^-1范围内线性关系良好(R2=0.9998),回收率为100.41%(RSD=1.42%,n=5),丹酚酸B在7.812—250.0μg·mL^-1范围内线性关系良好(R2=0.9992),回收率为100.77%(RSD=2.15%,n=5),其精密度、稳定性和平均加样回收率均符合含量测定要求。结论:本法简单易行,结果准确可靠,可有效地用于降脂合剂的质量控制。
Objective : To establish the HPLC method for determining the contents of 5 - HMF, atractydin and salvianolic acid B in Jiangzhi Mixture. Methods: HPLC assay was carried out on a column of Hibar ODS (4.6 mm × 250 mm,5 μm). The mobile phase was acetonitrile -0.1% formic acid solution with gradient elution at a flow rate of 1 mL · min^-1. The injection volume was 10 μL. The testing conditions were determined as detection wavelength in 300 nm and the column temperature at 35℃. Results: 5 - HMF, atractydin and salvianolic acid B showed good linear correlations in the range of 7. 812 - 250.0μg · mL^-1. The recycling rate of 5 - HMF, atractydin, salvianolic acid B was respectively 100.03 % ( RSD = 1.76%, n = 5 ), 100.41% ( RSD = 1.42 %, n = 5 ), 100.77% ( RSD = 2.15 %, n = 5 ). The precision, accuracy and recovery met the determination requirements. Conclusion: The method is simple, quick, reliable and accurate and very suitable to the quality control of Jiangzhi Mixture.
出处
《中华中医药学刊》
CAS
北大核心
2015年第10期2458-2461,共4页
Chinese Archives of Traditional Chinese Medicine
基金
无锡市卫生局面上项目(ML201306)
江阴市卫生局重点项目(Z201402)