HPLC-DAD法测定降脂合剂中5-羟甲基糠醛、苍术素、丹酚酸B的含量

Determination of 5-HMF and Salvianolic Acid B in Jiangzhi Mixture by HPLC

目的：建立降脂合剂中5-羟甲基糠醛（5-HMF）、苍术素（atractydin）、丹酚酸B（salvianolic acid B）的高效液相含量测定方法。方法：采用Hibar ODS柱（4．6mm×250mm，5μm），以乙腈-0．1％甲酸溶液为流动相梯度洗脱，流速为1．0mL·min^-1，检测波长为300nm，柱温为35℃，进样量10μL。结果：5-羟基甲基糠醛在7．812～250．0μg·mL^-1范围内线性关系良好（R。=0．9993），回收率为100．03％（RSD=1．76％，n=5），苍术素在7．812—250．0μg·mL^-1范围内线性关系良好（R2=0．9998），回收率为100．41％（RSD=1．42％，n=5），丹酚酸B在7．812—250．0μg·mL^-1范围内线性关系良好（R2=0．9992），回收率为100．77％（RSD=2．15％，n=5），其精密度、稳定性和平均加样回收率均符合含量测定要求。结论：本法简单易行，结果准确可靠，可有效地用于降脂合剂的质量控制。

Objective ： To establish the HPLC method for determining the contents of 5 - HMF, atractydin and salvianolic acid B in Jiangzhi Mixture. Methods： HPLC assay was carried out on a column of Hibar ODS （4.6 mm × 250 mm,5 μm）. The mobile phase was acetonitrile -0.1% formic acid solution with gradient elution at a flow rate of 1 mL · min^-1. The injection volume was 10 μL. The testing conditions were determined as detection wavelength in 300 nm and the column temperature at 35℃. Results： 5 - HMF, atractydin and salvianolic acid B showed good linear correlations in the range of 7. 812 - 250.0μg · mL^-1. The recycling rate of 5 - HMF, atractydin, salvianolic acid B was respectively 100.03 % （ RSD = 1.76%, n = 5 ）, 100.41% （ RSD = 1.42 %, n = 5 ）, 100.77% （ RSD = 2.15 %, n = 5 ）. The precision, accuracy and recovery met the determination requirements. Conclusion： The method is simple, quick, reliable and accurate and very suitable to the quality control of Jiangzhi Mixture.