针刺对大鼠腹部术后胃肠组织中NOS表达的影响

Effect of Acupuncture on the Expression of NOS in Gastrointestinal Tissues after Abdominal Operation in Rats

目的观察针刺对不全性肠梗阻大鼠术后胃肠组织中一氧化氮合酶(NOS)表达的影响,探讨针刺治疗促进胃肠运动功能紊乱恢复的机制。方法将40只SD大鼠随机分为治疗1组、治疗2组、模型1组、模型2组和空白组,每组8只。治疗组和模型组均采用丝线单纯结扎法造成不完全性肠梗阻模型。治疗组造模后采用针刺足三里和阳陵泉治疗,其中治疗1组和治疗2组分别治疗1、2 d后解剖及取材。模型1组和模型2组分别于造模1、2 d后解剖及取材。对大鼠肠组织进行形态学观察,并比较各组肠梗阻上段肠管平均周径和胃肠组织标本中NOS含量变化。结果模型组大鼠造模后回盲部肠管周径与空白组比较,差异均具有统计学意义(P<0.05)。模型2组回盲部肠管周径与模型1组比较,差异具有统计学意义(P<0.05)。治疗组治疗后回盲部肠管周径与模型组和治疗2组比较,差异均具有统计学意义(P<0.05)。治疗2组回盲部肠管周径与空白组比较,差异无统计学意义(P>0.05)。模型组肠NOS含量与空白组比较,差异均具有统计学意义(P<0.05)。治疗1组治疗后肠NOS含量与模型组和治疗2组比较,差异均具有统计学意义(P<0.05)。各组肠NOS含量比较,差异均无统计学意义(P>0.05)。结论针刺可以通过调节肠组织中NOS含量变化的途径,促进不全性肠梗阻术后大鼠胃肠运动功能的恢复。

Objective To observe the effect of acupuncture on the expression of nitricoxide synthase （NOS） in gastrointestinal tissues after operation for incomplete intestinal obstruction in rats, and to explore the mechanism of acupuncture in promoting the recovery of gastrointestinal motility. Methods Forty Sprague-Dawley （SD） rats were randomized into treatment group 1, treatment group 2, model group 1, model group 2, and a normal control group, 8 rats in each group. The incomplete intestinal obstruction models were developed by using single ligation method in treatment groups and model groups. Afterwards, the treatment groups were intervened by acupuncture at Zusanli （ST 36） and Yanglingquan （GB 34）, and rats in treatment group 1 and 2 were respectively dissected after 1-day and 2-day treatment. The rats in model group 1 and 2 were respectively dissected 1 d and 2 d after modeling. The morphological observation was performed on rat’s intestinal tissues, and the average diameter of the upper part of the obstructed intestine and the expression of NOS in gastrointestinal tissues were compared. Results The average ileocecal diameter after modeling was significantly different from that in the normal control group （P〈0.05）. There was a significant difference in comparing the ileocecal diameter between model group 1 and 2 （P〈0.05）. The ileocecal diameter in treatment group 1 was significantly different from that in treatment group 2 and model groups （P〈0.05）. The ileocecal diameter in treatment group 2 was insignificantly different from that in the normal control group （P〉0.05）. The NOS content in model groups was significantly different from that in the normal group （P〈0.05）. The NOS content in treatment group 1 was markedly different from that in model groups and treatment group 2 （P〈0.05） after intervention. There were no significant inter-group differences in comparing NOS content （P〉0.05）. Conclusions Acupuncture can promote the recovery of gastrointestinal motility by regulating intestinal NOS content in rats after operation for incomplete intestinal obstruction.