实时超声造影用于评价高强度聚焦超声治疗兔VX2肝癌模型效果的研究

Contrast enhanced ultrasound applied for evaluation of high intensity focused ultrasound in treatment of rabbit VX2 liver cancer model

[目的]探讨活体肝癌经高强度聚焦超声(HIFU)治疗前后实时超声造影(CEUS)定量参数的变化特点,及其与肿瘤微血管密度(MVD)的相关性。[方法]选取30只新西兰大白兔制成VX2肝癌模型,随机分为对照组(15只,不作HIFU治疗)和治疗组(15只,作HIFU治疗)。行CEUS检查,获得并记录曲线增强上升斜率(AS)、开始增强时间(AT)、达峰时间(TTP)、峰值强度(PI)、曲线下面积(AUC),并与造影后通过免疫组织化学检查获得的MVD进行相关性分析。[结果]治疗组AS、PI、AUC较对照组明显下降(P<0.05);MVD较对照组明显减少(P<0.05);AT、TTP较对照组明显延迟(P<0.05)。CEUS定量参数中AS、PI、AUC分别和MVD呈正相关(P<0.05);AT、TTP分别和MVD呈负相关(P<0.05)。[结论]肝癌组织的CEUS定量参数与MVD具有高度的相关性,可反映肝癌组织微血管结构变化的情况并有助于判断HIFU对肝癌的疗效及预后。

[ Objective ] To explore the variation features of contrast enhanced ultrasound （CEUS） quantitative parameters and its correlation with tumor microvessel density （MVD }, after the treatment of living liver cancer using high intensity focused ultrasound （ HIFU ）. [ Methods] A group of 30 New Zealand white rabbits were made into VX2 liver cancer models, that were randomly divided into control group （n = 15, not HIFU treatment） and treatment group （n = 15, HIFU treatment）. CEUS was performed, then obtaining ascending slope （ AS ）, arrival time （ AT ）, time-to-peak （ TrP ）, peak intensity （PI） ,area under curve （AUC）. And correlation analysis was performed with MVD obtained by immune histochemical method. [ Results ] Compared with those in the control group, AS, PI, AUC were significantly decreased in treatment group （ P 〈 0.05 ） and so was MVD （ P 〈 0.05 ）. AT, TIP were significandy delayed than those in the control group （ P 〈 0.05 ）. The AS, PI, AUC of CEUS quantitative parameters were positively correlated with MVD （P 〈 0.05 ）. The AT, TIP respectively showed a negative correlation with MVD （ P 〈 0.05 ）. [ Conclusion] CEUS quantitative parameters of liver cancer tissue have high correlation with MVD. It can reflect the microvessel structure change of the liver cancer tissue and help to judge the curative effect and prognosis of HIFU for liver cancer.