不同培养基对湖南汝城温泉微生物分离培养探究

Isolating Culture on Hunan Rucheng Hot Spring Microorganism with Different Medium

目的:探讨不同培养基对湖南汝城温泉微生物中可培养细菌的多样性.方法:用DSM88、牛肉膏蛋白胨、高氏一号三种培养基分别于50℃培养罗泉温泉和60℃培养热水温泉样品.用稀释涂布平板法和平板划线法分离、纯化嗜热菌并进行革兰染色和生理生化试验。结果:81个菌株中革兰氏染色阳性菌50株,阴性菌31株.DSM88、牛肉膏蛋白胨和高氏一号培养基中罗泉和热水样品分别获得的菌株数为23、15、3,25、6、9.结论:三种培养基中DSM88培养的嗜热菌种类最多;采用不同培养基丰富了可培养嗜热菌的资源.

The paper discusses Rucheng hot- spring microorganism educable bacteria diversity on different medium. Using three kinds of medium to cultivate hot spring microorganism,by way of DSM88 medium,Beef extract peptone medium and Gao＇s medium to cultivate of hot springs microbial,Warmspring to 50℃,hotspring to 60 ℃. By attenuating coating method and plating streak method,separating and purifying hot- spring thermophilic bacteria and makes experiment of Gram＇s staining and physiological- biochemical test. Moreover, do morphological observation and physic- chemical property experiment. All the isolated strains were gram positive. Separated twenty- three kinds of bacteria from warm- spring by DSM88 medium,five strains of staphylococcus aureus and eighteen of pole state; one strains of staphylococcus aureus and fourteen of pole state from beef extract peptone medium; three kinds of rod- shaped bacteria from Gao＇s medium. Twenty- five kinds of bacteria from hot spring with DSM88 medium and all are rod- shaped bacteria; six rod- shaped bacteria from beef extract peptone medium and nine kinds of gram positive rod- shaped bacteria from Gao＇s medium.DSM88 medium is a kind of normal medium using for hot- spring thermophilic bacteria,it can get more educable thermophilic bacteria resource with different mediums.