莱菔硫烷对大鼠缺血性脑卒中后血管新生的影响

Effect of Sulforaphane on Angiogenesis in Rats with Focal Cerebral Ischemia

目的:观察莱菔硫烷(SFN)对大鼠缺血性脑卒中后血管新生的影响。方法:36只SD大鼠随机分成假手术组、模型组和SFN组各12只。采用改良线栓法制作大鼠大脑中动脉缺血(MCAO)模型,假手术组不予缺血处理,SFN组腹腔注射SFN,模型组腹腔注射等量生理盐水。MCAO术后1、7、14 d进行神经功能评分;术后14 d采用免疫组化法检测梗死灶周围区二维微血管密度,术后14 d股静脉注射异硫氰酸荧光素右旋糖酐(FITC-dextran)标记微血管,分析梗死灶周围区三维微血管的直径、面积及分支数目。结果:术后1 d模型组和SFN组神经功能评分差异无统计学意义,术后7、14 d SFN组的神经功能评分低于模型组(P<0.01)。术后14 d模型组和SFN组的二维微血管密度显著高于假手术组(P<0.01),模型组和SFN组的三维微血管直径小于假手术组(P<0.05),面积及分支数目均高于假手术组(P<0.01)。SFN组二维微血管密度、三维微血管直径、面积和分支数目均高于模型组(P<0.05)。结论:SFN可促进缺血性脑卒中大鼠的神经功能恢复,其机制可能与促进缺血性脑卒中后微血管新生有关。

Objective： To observe the effect of Sulforaphane （SFN） on angiogenesis in rats with focal cerebral ischemia. Methods： Thirty-six SD rats were randomly divided into sham group （n=12）, model group （n=12） and SFN group （n=12）. The unilateral middle cerebral artery occlusion （MCAO） model was established by modified intraluminal filament method. The sham group was treated in the same manner as those undergoing MCAO, howev-er, no occluding thread was inserted. Rats in the SFN group were injected intraperitoneally with SFN whereas those in the model group received normal saline of equal volume. The modified neurologic severity score （mNSS） were evaluated at 1, 7 and 14 d after MCAO. Microvessel density in ischemic boundary regions was measured by immunohistochemistry at 14 d after MCAO. Microvessel diameters, vessel areas and numbers of branch points were analyzed at the ischemic boundary perfused with fluorescein isothiocyanate （FITC） dextran at 14 d after MCAO. Results： There was no difference in mNSS between the model group and SFN group at 1 d after MCAO, but the mNSS of SFN group were significantly lower than that of model group at 7 d and 14 d after MCAO （P〈0.01）. The model group and SFN group presented higher microvessel density （P〈0.01） as well as smaller microvessel diameters （P〈0.05） than those in the sham group. The vessel areas and numbers of branch points in the model group and SFN group were significantly higher than those in the sham group （P〈0.01）. The microvessel density, the microvessel diameters, vessel areas and numbers of branch points in the SFN group were significantly higher than those in the model group （P〈0.05）. Conclusion： SFN could promote angiogenesis in ischemic boundary regions and exert cerebral protection.