高压氧对大鼠脊髓损伤的治疗作用及机制

Therapeutic effect of hyperbaric oxygen on spinal cord injury in rats and its mechanism

目的观察高压氧(HBO)治疗对大鼠脊髓损伤(SCI)的治疗作用,并探讨其可能机制。方法 25只健康清洁级成年SD大鼠随机分为SCI组10只、HBO组10只、对照组5只,SCI组、HBO组大鼠均制备SCI模型,HBO组制模成功后采用HBO治疗;对照组采取同前方法咬开椎板,不损伤脊髓,后给予缝合。观察两组大鼠不同时点(制模成功后6 h及1、3、7、14、21 d,分别计为T1、T2、T3、T4、T5、T6)BBB评分及脊髓组织中突触素ⅠmRNA。结果与对照组比较,HBO组、SCI组各时点(T3、T4、T5、T6)BBB评分及脊髓组织突触素ⅠmRNA表达升高(P均<0.05);与SCI组比较,HBO组各时点(T3、T4、T5、T6)BBB评分及脊髓组织突触素ⅠmRNA表达升高(P均<0.05)。结论 HBO可促进大鼠损伤脊髓的恢复,其机制可能与上调损伤脊髓组织内突触素ⅠmRNA表达有关。

Objective To observe the therapeutic effect of hyperbaric oxygen （ HBO ） on the spinal cord injury （ SCI） , and to explore its possible mechanism.Methods Twenty-five healthy and clean SD adult rats were randomly di-vided into the SCI group （n=10）, HBO group （n=10） and the control group （n=5）.The SCI model rats were prepared in the SCI and HBO groups, after the success of molding, the HBO group received HBO treatment;the vertebral plates of rats in the control group were opened and sutured without damage to the spinal cord.BBB rating and synaptophysinⅠmR-NA in the spinal cord tissues at different time points （6 h after molding and 1, 3, 7, 14 and 21 d, which is counted as T1 , T2 , T3 , T4 , T5 and T6 ） were observed in the two groups.Results Compared with the control group, the BBB rating and synaptophysinⅠmRNA expression was increased in the HBO group and SCI group at each time point （ T3 , T4 , T5 and T6 ） （all P〈0.05）.Compared with the SCI group, the BBB rating and synaptophysin Ⅰ mRNA expression was increased in the HBO group at each time point （T3, T4, T5 and T6） （all P〈0.05）.Conclusion HBO promoted the recovery of the injured spinal cord of rats, which may be related with the up-regulation of synaptophysin Ⅰ mRNA expression in the in-jured spinal cord tissues.