甘草次酸脂质体对OVA免疫小鼠免疫应答的影响

Adjuvant effect of glycyrrhetinic acid liposome on the immune responses to ovalbumin in mice

为了观察甘草次酸被脂质体包封后其免疫增强作用的变化,选取100只小鼠,随机分成4组,分别用含有甘草次酸脂质体(GAL)、甘草次酸(GA)、空白脂质体(BL)和油佐剂(OIL)的卵清蛋白(OVA)免疫小鼠。采用ELISA法测定用OVA免疫的小鼠血清中免疫球蛋白Ig G及细胞因子IL-2,IFN-γ的浓度变化;采用MTT法测定脾淋巴细胞的增殖。结果表明,GAL能显著增加Ig G,IL-2和IFN-γ的浓度,促进淋巴细胞增殖,且效果好于GA,BL或油佐剂。表明GA经脂质体包封后,二者可协同增强免疫。

In order to study the action mechanism of glycyrrhetinic acid liposome （GAL） , 100 mice were vaccinated by ovalbumin along with glycyrrhetinic acid liposome （ GAL）, glycyrrhetinic acid （ GA ）, liposome （ BL ） or oil （OIL） in four groups. Blood samples were collected to measure antibody titers, IL-2 and IFN-γ by ELISA assay; cell mediated immune response was detected by MTF assay. The results showed that GAL could improve the secretion of IgG, IL-2 and IFN-γ, and significantly promote lymphocyte proliferation in comparison with glycyrrhetinic acid, liposome and oil. The present study demonstrated that the activity of glycyrrhetinic acid encapsulated with liposome was enhanced.