番茄类胡萝卜素合成基因鉴定及表达分析

Identification and expression analysis of carotenoid biosynthesis genes in tomato

类胡萝卜素是光合组织中一类高抗氧化性的脂溶性物质,番茄是人类获取类胡萝卜素的重要蔬菜之一。该研究主要基于番茄全基因组测序信息,利用生物信息学方法对番茄类胡萝卜素合成酶基因进行鉴定及表达分析。结果显示,番茄中共鉴定了47个类胡萝卜素合成酶基因,不均匀分布在除9号染色体外的其余11条染色体上;这些结果为我们进一步揭示类胡萝卜素代谢基因的进化关系提供了信息基础;RNA-Seq分析表明这些基因在番茄栽培种不同组织中表达,包括根、茎、叶、花和果实,表明这些基因在番茄营养生长和生殖生长过程中具有重要作用。

Carotenoids, a group of high oxidation of fat-soluble substances in photosynthetic tissues, are known to be abundance in tomato. Tomato is one of the important vegetables all over the world, including China. Research on genes involved in their biosynthesis can provide further information regarding the molecular mechanism in divergent tissues. In this study, identification and expression analysis of carotenoid biosynthesis genes in tomato were per- formed by bioinformatics methods based on tomato whole genome sequencing information. Results showed that a total of 47 carotenoid biosynthesis genes were identified, which were distributed unevenly on all these chromosomes, other than chromosome 9. Identification, genomic organization and phylogenetic analysis would help revealing the potential mechanisms responsible for the evolution of genes within and among species. RNA-Seq analysis showed that these genes were involved in different tissues of the cultivated tomato, including root, stem, leaf, flower and fruit, sugges- ting an important role in vegetative and reproductive growth in tomato.