应用TDZ优化生菜再生体系的建立

Establishment and optimization of the regeneration system for lettuce using TDZ

以生菜（Lactuca sativa L.）栽培品种香港玻璃生菜为试验材料建立了高频再生快繁体系,研究了不同消毒方式对种子消毒效果及无菌苗生长的影响,探讨了不同激素水平和培养基对子叶愈伤组织诱导、再分化及诱导生根的关系。结果如下：种子在70%酒精处理60 s,2%Na Cl O消毒10 min后,播种到MS培养基上种子发芽率最高,达98%,且无菌苗生长好;外植体宜选生长3~5 d的生菜无菌苗子叶,适宜不定芽分化的最优条件组合为MS＋0.1 mg·L-1TDZ＋0.1 mg·L-1NAA,不定芽分化率高达83.3%,分化指数为7.7,MS＋0.3 mg·L-16-BA＋0.1 mg·L-1NAA适宜其继代扩繁培养;NAA和IAA复合使用有利于生根,最适生根培养基为1/2MS＋0.05 mg·L-1NAA＋0.5 mg·L-1IAA。

Lettuce cuhivar Hongkong glass lettuce was used as the test material to establish a high frequency regener- ation and rapid propagation system. Effects of different sterilization methods on disinfection of seed, the growth of seedling, and the relationship between different hormones and medium on the induction callus of cotyledon, differen- tiation and rooting were studied. The results were as follows ： When the lettuce seeds were treated for 60 s with 70% ethanol, followed by 2% NaC10 for 10 min, the germination rate reached 98%, and the sterile seedlings grew well on MS medium. 3 - 5 d cotyledons should be selected as explants, and the optimal condition of adventitious buds was MS medium combined with 0. 1 mg.L-1 TDZ and 0. 1 mg.L-1 NAA while the differentiation rate reached 83.3% and differentiation index was 7.7. The medium of MS ＋ 0. 3 mg. L- 16-BA ＋ 0. 1 mg. L- 1 NAA was optimal for the fol- lowing generation and proliferation culture. The combination of NAA and IAA was beneficial for rooting, and the op- timal medium for rooting was 1/2MS medium with 0. 05 mg.L-1 NAA and 0. 5 mg.L-1 IAA.