铁皮石斛种子组织培养条件的优化

Optimization of tissue culture conditions for Dendrobium candidum seeds

以铁皮石斛种子为材料,通过组织培养技术,对比原球茎和丛生芽的增殖能力,并比较种子消毒方式、琼脂、有机物、活性炭等添加物对铁皮石斛丛生芽增殖和壮苗生根的影响,优化前期铁皮石斛组培技术体系。结果显示:原球茎增殖能力远高于丛生芽的增殖能力且增殖速度快;铁皮石斛种子消毒方式以75%乙醇(浸泡30 s)+8.0%次氯酸钠(浸泡8 min)效果较好,生产中可取代传统升汞溶液消毒方式;液态培养基显著优于半液态和固态培养基;培养基中添加10%香蕉汁和0.5%活性炭有利于芽苗的壮苗生根。

The seed of Dendrobium candidum was used as test materials to compare the effects of seed disinfection methods, agar, organic compound and activated carbon on adventitious buds proliferation and shoots strengthening and roots induction in tissue culture, in order to optimize the preliminary system for the tissue culture of Dendrobium candidum. The results showed that the proliferation of protocorm-like bodies was higher than muhiple shoots. The disinfection method of 75 % alcohol （ for 30 s） ＋ 8. 0% NaClO （for 8 min） was optimum for seeds, which could re- place the traditional method of 0. 1% HgCl2. The liquid medium was superior to semi liquid and solid culture medi- um. Addition of 10% banana juice and 0. 5% activated carbon in culture medium could promote the growth of shoots and roots.