狂犬病病毒RT-PCR检测方法的建立

Method of RT-PCR for Detection of Rabies Virus

为建立一种检测狂犬病病毒的方法,根据Gen Bank已公布的狂犬病病毒核蛋白N基因序列,设计并合成一对特异性的引物,以兽用疫苗株HEP-Flury为阳性毒株建立了RT-PCR检测方法。对建立的RT-PCR方法做灵敏度、特异性、重复性及稳定性试验,结果显示该方法的灵敏度可以达到20 FFU/m L,可以将狂犬病病毒与犬瘟热病毒和犬细小病毒区别开来。该方法重复性好,稳定可靠。

To establish the method of RT-PCR for detection of Rabies virus,a pair of primers were designed based on nucleoprotein sequences of Rabies virus that published in GenBank. RNA extracted from Rabies virus vaccine HEP-Rlury was as standard positive control. The results showed that the method could distinguish Rabies virus from canine distemper virus,canine parvovirus.The sensitivity could attained 20 FFU/mL and the stability was good.