甘肃省苹果树腐烂病菌ITS序列分析及潜育期研究

Study on the incubation period and ITS sequence of pathogen of apple tree Valsa canker in Gansu Province

为了明确甘肃省苹果树腐烂病菌的种类及其优势种的潜育期,通过ITS序列比对和离体枝条接种法对采自甘肃省不同地区的苹果树腐烂病菌进行了ITS序列测定、分析及潜育期研究.结果表明:甘肃省苹果树腐烂病菌有2个种,分别为Valsa mali和Valsa malicola,其中Valsa mali有2个变种,分别为Valsa mali var.mali和Valsa mali var.pyri.甘肃省苹果树腐烂病菌优势种Valsa mali的潜育期随保湿时间增加而缩短,保湿时间为72h时潜育期为74.3h;温度30℃时潜育期最短,为64.3h;潜育期随枝龄的增加而增加,嫩梢的潜育期为46.3h;光暗交替下潜育期最短,为45.3h.

In order to clarify incubation period and pathogen of apple tree Valsa canker in Gansu Prov- ince, the ITS sequence and incubation period of pathogen were studied by analysis ITS sequence and excised twigs inoculating. The results showed that there were two species of Valsa could cause this disease in Gan- su,Valsa rnali and Valsa rnalicola. The V. rnali could be also divided into two varieties,V, mall vat. mall and V. rnali var. pyri. Incubation period of apple Valsa canker shortened with the increasing of moisture time. When moisture time was 72 h, the incubation period was 74. 3 h. The incubation period was the shor- test at 30 ℃. The incubation period also prolonged when branch age increased, the incubation period of young shoots was 46.3 h. The incubation period under the alternation of light and darkness every 12 h was the shortest,that was 45.3 h