A型产气荚膜梭菌tetB(P)耐药基因核苷酸序列与蛋白结构分析

Sequence and protein analysis on the tetB(P) resistance gene of Clostridium perfringens type A

为了对产气荚膜梭菌tetB(P)耐药基因进行分析,通过生物软件设计引物,扩增A型产气荚膜梭菌tetB(P)耐药基因,测序后对tetB(P)基因核苷酸序列与蛋白结构进行分析.结果表明:分离株tetB(P)基因长度为1 959bp,编码652个氨基酸.与参考菌株CW92、EHE-NE18的核苷酸序列同源性依次为99.7%、99.9%,氨基酸序列同源性依次为99.4%、99.8%.分离株tetB(P)蛋白亲水性氨基酸较多,无跨膜区,含有4个N-糖基化位点,1个cAMP和cGMP依赖性蛋白激酶磷酸化位点,9个蛋白激酶C磷酸化位点,12个酪蛋白激酶Ⅱ磷酸化位点,7个N-豆寇酰化位点,1个ATP/GTP结合位点基序,1个翻译型鸟苷酸结合域,三级结构主要是由α-螺旋、β折叠和无规则卷曲形成.

In order to analysis tetB（P） drug resistance gene of Clostridium perfringens,primers were designed by biology software and were used to amplified tetB（P） gene of Clostridium perfringens type A, the amplified gene was sequenced and the protein structure was analyzed. The results showed that the tetB （P） gene was 1 959 bp long,encoding 652 amino acids. Nucleotide sequence homology of tetB（P） gene with reference strains CW92 and EHE-NE18 were 99. 7% and 99. 9%, respectively, amino acid sequence homolo- gy were 99.4% and 99.8%. The tetB（P） of isolated strain had more hydrophilic amino acid, no transmem- brane domain, had four N-glycosylation sites, one cAMP and cGMP dependent protein kinase phosphoryla- tion site, nine protein kinase C phosphorylation sites, twelve Casein kinase Ⅱ phosphorylation sites, seven N-myristoylation sites, one ATP/GTP-binding site motif A, one translational （tr）-type guanine nucleotide- binding （G） domain signature.