肥大细胞对前列腺癌细胞上皮间质转化的作用

The role of mast cells in epithelial-mesenchymal transition of prostate cancer cells

目的利用体外细胞共培养模型,探讨肥大细胞对前列腺癌细胞增殖及侵袭、转移的作用。方法选取肥大细胞瘤P815细胞系及前列腺癌LNCa P细胞,使用24孔Transwell小室构建2种细胞体外共培养模型。四甲基偶氮唑蓝(MTT)比色试验检测前列腺癌LNCa P细胞的增殖,反转录定量聚合酶链反应(q RT-PCR)方法和蛋白印迹法检测前列腺癌LNCa P细胞E-cad、N-cad、Vimentin的表达。结果前列腺癌LNCa P细胞与不同浓度肥大细胞共同培养12 h后吸光度(A)值变化与对照组差异无统计学意义(P>0.05),共同培养24 h及48h后A值显著高于对照组,差异有统计学意义(P<0.05);与对照组比较,实验组E-cad表达明显减弱;N-cad、Vimentin表达增强,差异有统计学意义(P<0.05)。结论肥大细胞可促进前列腺癌细胞的增殖并促进前列腺癌细胞的上皮间质转化,可能促进前列腺癌细胞侵袭、转移。

Objective To explore the role of mast cells in proliferation, invasion and metastasis of prostate cancer cells by using in-vitro cell co-culture model. Methods In-vitro co-culture model of mastocytoma P815 cell lines and prostate cancer LNCap cells was constructed by using 24-well Transwell chamber. Methylthiazolyl tetrazoli-um blue （MTT） colorimetry was used to detect the proliferation of prostate cancer LNCap cells. Quantitative reverse transcription polymerase chain reaction （qRT-PCR） and Western blotting were used to detect the expression of E-cad, N-cad and Vimentin in prostate cancer LNCap cells. Results Compared with the control group, the absorbance （A value） of prostate cancer LNCap cells co-cultured with different concentrations of mast cells was not significantly dif-ferent at 12h （P〉0.05）, but was significantly higher at 24h and 48h （P〈0.05）. The experimental group showed signifi-cantly down-regulated E-cad expression and up-regulated N-cad and Vimentin expression, with significant difference compared with the control group （P〈0.05）. Conclusion Mast cells can promote the proliferation and the epithelial-mesenchymal transition of prostate cancer cells, and may also promote the invasion and metastasis of prostate cancer cells.