摘要
目的观察成人隐匿性自身免疫性糖尿病(LADA)患者不同血糖水平对CD4+CD25+调节性T细胞以及白细胞介素(IL-10)的影响。方法病程5年以内的LADA患者104例,根据患者糖化血红蛋白(Hb A1C)水平以及个人意愿分为四段胰岛素强化治疗组A组、B组和预混胰岛素治疗C、D组;于治疗前及治疗2w和4w后分别检测空腹血糖(FBG)、Hb A1C及空腹C肽;以流式细胞技术测定外周血CD4+CD25+调节性T细胞数量;以双抗体夹心ELISA法检测IL-10水平。结果治疗前A、C组患者CD4+CD25+调节性T细胞以及IL-10略高于B、D组患者,但差异无统计学意义(P>0.05);胰岛素强化治疗2w后、四组患者胰岛细胞功能较治疗前好转、CD4+CD25+调节性T细胞及IL-10较治疗前升高,顺序依次为A>B>C>D;实验进行至第4w,A、B组患者胰岛细胞功能持续得到改进、CD4+CD25+调节性T细胞及IL-10进行性上升。结论 LADA患者经过胰岛素强化治疗后血糖达标可使其胰岛细胞功能较治疗前恢复;CD4+CD25+调节性T细胞以及其IL-10升高。
Objective To investigate the effects of regulatory T cells and IL-10 in patients with 1 Methods There were 104 cases of LADA within tensive therapy group （group A and B）and non- in different blood glucose levels on CD4＋CD25＋ atent autoimmune diabetes in adults （LADA）. five years of LADA, they were divided to in- tensive therapy group （group C and D） accord-ing to their choice. Before treatment and after two weeks and four weeks, fasting blood glucose （FBG）, glycosylated hemoglobin （HbA1C） and islet cell function （fasting C-peptide）were detected respectively. Determine the lymphocytes subpopulations （CD4＋ CD25~ T cell） in peripheral blood mononuclear cells by flow cytometry. Levels of IL-10 peripheral blood were assessed by ELISA. Results The function of islet was progressed in group A and the rate of CD4~CD25~ regulatory T cells and the levels of IL-10 were increased by the 2-weeks insulin intensive therapy on group A and B but group C or D. As the experiment proceeds to four weeks, patients with islet cell function continuously improved, CD4＋CD25~ regulatory T cells and the levels of IL-10 increased progressively. Conclusions Patients with LADA treated by insulin intensive may make the blood glucose standardization and ameliorate tl^e immunodeficiency by increasing the rate of CD4~ CD25~ T cell and and the levels of IL-10,and then improve the function of islet.
出处
《现代诊断与治疗》
CAS
2015年第16期3605-3607,共3页
Modern Diagnosis and Treatment
