胆囊癌中趋化因子CXCL12和CXCR4的表达

The expression of chemokine CXCL12 and CXCR4 in gallbladder cancer

目的研究原发性胆囊癌中趋化因子CXCL12和CXCR4的表达并讨论其临床其意义。方法选择2010-01~2014-12手术切除病理证实胆囊癌标本132例,其中男性74例,女性58例,选择24例正常胆囊作为对照。用免疫组织化学方法测定在胆囊癌和正常胆囊中CXCL12和CXCR4的表达。结果 CXCL12和CXCR4表达阳性染色均定位于胆囊癌细胞胞质。CXCL12和CXCR4表达在胆囊癌中的阳性表达率分别为69.7%（92/132）和62.1%（82/132）,而正常胆囊表达分别为16.7%（4/24）和8.3%（2/24）,胆囊癌中两者表达明显高于正常胆囊（P均〈0.01）。CXCL12和CXCR4表达与Nevin分期、淋巴结转移密切相关（P〈0.05）。结论 CXCL12和CXCR4在胆囊癌中有明显表达,且与Nevin分期、淋巴结转移密切相关,提示测定它们的表达对原发性胆囊癌的诊断具有一定临床意义。

Objective To investigate the expression of CXCL12 and CXCR4 in gallbladder cancer, and to explore its clinical signifi- cance. Methods From January 2010 to December 2014,132 cases of surgically confirmed gallbladder cancer were selected, 74 males and 58 females, and 24 cases of normal gallbladder were taken as controls. Expression of CXCL12 and CXCR4 was determined by im- munohistochemistry in gallbladder cancer and normal gallbladder. Results The positive CXCL12 and CXCR4 cells were expressed in gallbladder cancer cytoplasm. The positive rates of CXCL12 and CXCR4 expression were 69.7% （92/132） and 62.1% （82/132） in gallbladder cancer, and 16.7% （4/24） and 8.3 % （2/24） in normal gallbladder, respectively, and the expression in gallbladder canc- er was significantly higher than that in normal gallbladder（P 〈 0.01 ）. The expression of CXCL12 and CXCR4 was closely related with Nevin stage and lymph node metastasis（P 〈 O. 05）. Conclusion The results suggest that the CXCLI2 and CXCR4 expression may have some clinical significance for the diagnosis of primary gallbladder cancer.