PAA-CS超吸水海绵应用于喂服华法林大鼠拔牙创模型的止血效果

PAA-CS superabsorbent sponge used for hemostasis of tooth extraction wounds in rats fed on warfarin

目的探讨PAA-CS超吸水海绵应用于口腔拔牙创的优势。方法建立对照组(A组)SD大鼠拔牙创模型和华法林组(B组)SD大鼠凝血功能异常拔牙创模型,以纱布棉球做阴性对照对拔牙创进行止血,比较PAA-CS超吸水海绵与纱布棉球的止血效果。结果 B组凝血酶原时间(s)较A组显著延长[(19.73±2.74)vs(10.04±0.95),P<0.05],INR值增大(3.49 vs 1)。A、B两组中,PAA-CS超吸水海绵止血时间均少于纱布棉球,差异有统计学意义(P<0.05);A组止血后无继发出血;B组应用纱布棉球止血后有4只继发出血。结论成功建立了因喂服华法林而导致凝血功能异常的大鼠拔牙创模型,PAA-CS超吸水海绵的止血效果明显优于纱布棉球。

Objective To observe hemostatic effects of PAA-CS（ poly（ acrylic acid）-chitosan） superabsorbent sponge on SD rats fed on warfarin after their tooth extraction,discussing the advantages of PAA-CS superabsorbent sponge applied for tooth extraction wounds. Methods Group A,a model of tooth extraction wounds of SD rats,and Group B,a model of tooth extraction wounds of SD rats with coagulation function abnormality,were established to carry out hemostasis on tooth extraction wounds with a negative control group using gauze tampons. Hemostatic effects of PAA-CS superabsorbent sponge and gauze cotton balls were compared. Results By four coagulation tests,the coagulation function of rats in Group B became abnormal. During the examination of hemostasis of tooth extraction wounds,the time used for hemostasis by the PAA-CS Superabsorbent Sponge（ about 60 s） was significantly less than that by gauze tampons（ 〉90 s,P〈 0. 01）. After the PAA-CS superabsorbent sponge was used to stop bleeding of tooth extraction wounds in the bleeding model of rat tooth extraction wounds,there was no secondary hemorrhage and its hemostatic effect was reliable. Conclusions This examination established a model of tooth extraction wounds of rats fed on warfarin resulting in abnormality of rats＇ coagulation function successfully. The hemostatic tests have proved that the hemostatic effect of PAA-CS superabsorbent sponge is much better than that of gauze cotton balls.