直线加速器X线照射对人肺成纤维细胞经典Wnt通路中关键信号蛋白的影响

Effect of X-ray linear accelerator on the signaling proteins of canonical Wnt pathway in human lung fibroblasts

目的探讨直线加速器X线照射对人肺成纤维细胞经典Wnt通路中关键信号蛋白的影响。方法人肺成纤维细胞分为照射组和正常对照组,照射组经直线加速器X线5 Gy照射24 h后,Western blot检测两组成纤维细胞胞核内β-catenin蛋白表达,Real time PCR检测成纤维细胞胞核内β-catenin mRNA表达,ELISA法检测细胞培养液中WISP-1蛋白表达。结果照射组人肺成纤维细胞β-catenin蛋白表达和mRNA表达水平均高于对照组,WISP-1蛋白表达水平也显著升高,与对照组比较均有统计学意义[(637±88)vs(387±58),P<0.05]。结论直线加速器X线照射可诱导人肺成纤维细胞经典Wnt通路活化,促进肺纤维化的形成,可能为放射性肺损伤的治疗提供新的靶点。

Objective To investigate the effect of irradiation with X-ray linear accelerator on the key signaling proteins of canonical Wnt pathway in human lung fibroblasts（ HLFs）. Methods HLFs were divided into irradiated group and normal group. R group was irradiated by linear accelerators X-ray（ 5 Gy）. After 24 hours,the expression of the protein levels of β-catenin in nucleus was examined by Western blot,the mRNA level of the HLFs was examined by real time PCR,and the levels of WISP-1 protein in conditioned medium（ CM） was examined by ELISA. Results Compared with the normal control group,the protein and gene expression level of beta-catenin increased in irradiated group HLFs,the same to the protein expression levels of WISP-1,which were statistically significant（ P〈0. 05）. Conclusion Irradiation with X-ray linear accelerator can induce the activation of canonical Wnt signaling pathway in HLFs and promote the formation of pulmonary fibrosis,which probably provide the new target of the treatment of radiation-induced lung injury.