摘要
目的 探讨乌司他丁对烫伤大鼠脑组织的保护作用及其可能机制.方法 40只雄性SD大鼠按随机数字表法随机分为单纯烫伤组和乌司他丁组各20只,均造成50％总体表面积Ⅲ度烫伤,烫伤后即刻分别静脉注射生理盐水和乌司他丁4万U/kg.于烫伤后6h和24h断脑取材,用酶联免疫吸附（ELISA）法检测脑组织神经元特异性烯醇化酶（NSE）和热休克蛋白70（HSP70）的含量,Western印迹法测定脑组织中乙酰化组蛋白H3赖氨酸9（Ac-H3K9）表达,干／湿重法测定脑组织含水率.结果 伤后6h乌司他丁组脑组织匀浆中NSE含量和脑组织含水率均显著低于单纯烫伤组[（146＆#177;11）比（156＆#177;13） pg/ml和（77.3＆#177;1.9）％比（79.0 ＆#177;2.2）％],HSP70含量和Ac-H3K9相对表达量均显著高于单纯烫伤组[（99士19）比（92＆#177;13） pg/ml和（1.26＆#177;0.37）比（0.57＆#177;0.23）]（均P＜0.05）.伤后24 h乌司他丁组NSE含量显著低于单纯烫伤组[（141＆#177;14）比（159＆#177;10） pg/ml,P＜0.05],而含水率、HSP70含量、Ac-H3K9表达水平差异均无统计学意义[（75.9＆#177;1.2）％比（76.5＆#177;1.4）％、（118＆#177;17）比（102＆#177;16） pg/ml、（2.31＆#177;0.27）比（1.87＆#177;0.31）,均P＞0.05].结论 乌司他丁能减轻致死性烫伤大鼠早期的脑组织损害,可能与其能增加脑组织保护性蛋白表达有关.
Objective To explore the protective effects of ulinastatin on cerebral tissue in rats following lethal burn injury and its relationship with the expression of protective protein.Methods A total of 40 male Sprague-Dawley rats were randomly divided into scald and ulinastatin groups （n =20 each）.Both groups were subjected to 50% total body surface area third-degree burns.Immediately after scalding,1 ml normal saline was injected intravenously in scald group while 1 ml saline containing ulinastatin （40 000 U/kg） in ulinastatin group.Then cerebral tissue was harvested for measuring the activities of neuron-specific enolase （NSE） by enzyme-linked immunosorbent assay （ELISA） at 6 h and 24 h postinjury.And the level of acetylated histone H3 was detected by Western blot and heat shock protein 70 （HSP70） by ELISA.The rates of tissue water content in heart were determined by dry/wet weight.Results NSE and water content of cerebral tissue after 6 h injury in ulinastatin group were significantly lower than those in scald group （（146±11） vs （156±13）pg/ml,（77.3 ±1.9）% vs （79.0 ±2.2）%,both P〈 0.05）,the expressions of HSP70 and histone H3 after 6 h injury in ulinastatin group was significantly higher than those in scald group （（99 ± 19） vs （92 ± 13） pg/ml,（1.26 ±0.37） vs （0.57 ±0.23）,both P 〈 0.05）.The NSE content of cerebral tissue after 24 h injury in ulinastatin group was significantly lower than that in scald group （（141 ± 14） vs （159 ± 10） pg/ml,P 〈 0.05）.And the water content of cerebral tissue,the expressions of HSP70 and histone H3 after 24 h injury had no significant inter-group differences （（75.9 ±1.2）% vs （76.5 ±1.4）%,（118 ±17） vs （102±16） pg/ml,（2.31 ±0.27） vs （1.87 ±0.31）,both P 〉 0.05）.Conclusions Ulinastatin significantly alleviates cerebral tissue injury in early stage of burn injury.And its protective effects may be due to the up-regulations of histone H3 and HSP70.
出处
《中华医学杂志》
CAS
CSCD
北大核心
2015年第36期2952-2954,共3页
National Medical Journal of China
基金
空军后勤科研计划（CKJ12J027）
哈尔滨市科技创新人才研究专项资金（RC2015QN004096）
