2型糖尿病视网膜病变与DNA氧化损伤的关系

Relationship between diabetic retinopathy and oxidative damage to DNA

目的了解2型糖尿病合并糖尿病视网膜病变(diabetic retinopathy,DR)患者中机体DNA氧化损伤水平。方法选择87例2型糖尿病患者,采用眼底检查方式明确是否患有视网膜病变,然后将其分为糖尿病DR组和无DR组,DR组31例,其中男11例,女20例,平均(67.68±6.61)岁,无DR组56例,其中男24例,女32例,平均(65.91±3.91)岁,同时选择65例正常体检者为对照组。测定血清单核细胞DNA氧化损伤指标8羟基脱氧鸟嘌呤(8-OHd G)水平,比较3组之间血清8-OHd G水平。结果糖尿病组较对照组血8-OHd G水平升高,2组有统计学差异[(3.23±1.86)ng/ml比(0.72±0.93)ng/ml,t=9.972,P<0.001],DR组较无DR组血8-OHd G水平升高,2组间有统计学差异(t=2.441,P=0.017)。Speaman相关性分析显示,患者视网膜病变的严重程度与血清8-OHd G水平呈正相关。结论 2型糖尿病患者机体DNA氧化损伤指标8-OHd G水平增高,合并DR的患者,8-OHd G水平增高更明显,而且与DR的病变程度呈正相关,DNA氧化损伤可能不仅参与糖尿病发病,还与糖尿病的微血管并发症的发病有关。

Objective To investigate the level of DNA oxidative damage in the patients with diabetic retinopathy（ DR）. Methods Eighty-seven patients with type 2 diabetes mellitus（ T2DM） were enrolled in this study. According to the results of fundus photo detection,the patients were divided into DR group（ 31 cases,11 males and 20 females,mean age 61. 68 ± 11. 61 years） and non-DR group（ 56 cases,24 males and 32 females,mean age 60. 91 ± 13. 91 years）. Sixtyfive normal healthy were enrolled as control group. The level of serum 8-hydroxy-2’-deoxyguanosine（ 8-OHd G） was detected and compared between three groups. Results The level of 8-OHd G of the patients with T2 DM was higher than that of the normal healthy（ 3. 23 ± 1. 86 ng / ml vs. 0. 72 ± 0. 93 ng / ml,t = 9. 972,P 〈 0. 001）. The level of 8-OHd G of DR group was higher than that of non-DR group（ t = 2. 441,P = 0. 017）. Spearman analysis showed that the level of 8-OHd G was correlated with the severe degree of DR. Conclusions Oxidative damage of DNA is significantly higher in the patients with T2 DM,especially in the patients with DR. It suggests that oxidative damage of DNA might participate in the development of T2 DM and the microvascular disease,which provides reference for the anti-oxidative therapy for T2 DM with DR.