脂联素微球复合 PLGA/β-TCP 支架材料的制备及生物活性评价

Preparation and in vitro release behavior of adiponectin microsphere-scaffold composite

目的：制备载脂联素缓释骨支架材料，并评价其体外生物学性能。方法：利用离子乳化交联法制备负载脂联素的壳聚糖微球，应用热致相分离法制备乳酸和乙醇酸共聚物／β-TCP 支架材料并在其中包覆载药微球。通过扫描电子显微镜、体外释放行为、检测材料浸提液对 MC3T3细胞凋亡和增殖作用等实验综合评价载药支架材料的性能及生物学活性。结果：微球直径均匀，载药支架孔径20～200μm 并相互穿通，载药率1．3％，包封率70．3％，在缓冲液中药物释放持续91 d。材料浸提液诱导细胞凋亡率与空白对照组相当，载药支架材料对成骨细胞的增殖有促进作用。结论：载脂联素缓释骨支架材料具有缓释效果并促进成骨细胞增殖。

Objective：To prepare a bone substitute using microsphere scaffold containing adiponectin（APN）and to investigate the release behavior of the scaffold in vitro.Methods：Chitosan microsphere was developed by an emulsion-ionic cross-linking method. Poly （L-lactic-co-glycolic）acid （PLGA）and β-tricalcium phosphate （β-TCP）were used to prepare microsphere scaffold containing APN.The morphology,particle size,drug loading,incorporation efficiency and release behavior of the microsphere were examined. Results：The APN containing microsphere showed good spherical geometry,suitable size and microporosity under scanning electron microscope.The average diameter of the milipore was 20 -200 μm;the drug loading and incorporation efficiency were 1 .3% and 70.3% respectively.The controled-release process continued for 91 days.The extract solution from the APN microsphere-scaffold promoted MC3T3 cell proliferation without cytotoxicity.Conclusion：The APN microsphere-scaffold has sustained release function and may promote osteoblast proliferation.