响应面法优化酶解核桃多肽提取工艺

Optimization of Extracting Polypeptide from Walnut by Response Surface Methodology

近年来，随着贵州省农业产业结构调整，核桃产业得到迅猛发展，2013年，全省约有核桃140万亩，总产量1.3万吨，预计今年达到1000万亩，年产50万吨，已初步形成了我国具有较好发展前景的优质核桃产业带，成为贵州省重要的经济作物。

Walnut polypeptide was obstained by enzymatic hydrolysis with the defatted walnut meal as raw material.The preparation process of walnut polypeptide was optimized on foundation of single factor experiment , according to Box-Behnken central composition design principle ,the method of response surface analysis with 4 factors and 3 levels combined with Design Expert8.0.6-statistical data analysis software was adopted with the leaching rate of walnut polypeptide as the response value. The effect of substrate concentration, temperature, time and pH on the leaching rate of walnut polypeptide were studied in this paper. According to the above results, the optimum preparation conditions were obtained as follows： substrate concentration 2.3 % , temperature 51 ℃,time 5.2 h, and pH 6.5.Under these conditions, the leaching rate of walnut polypeptide reached 0.45 g/g.