长白山松子降血压肽制备工艺优化

Preparation and Optimization of Antihypertensive Peptides from Pinus koraiensis Sieb.et Zucc Nuts in Changbai Mountain

采用碱性蛋白酶对长白山松子分离蛋白进行酶解,以水解度和酶解物的ACE抑制率为考察指标,考察酶解p H、酶解温度、底物浓度、加酶量4个因素的影响,通过响应面(Box-Benhnken)组合优化试验,确定得到ACE抑制率最佳的条件为酶解p H8.9,酶解温度55℃,加酶量7 500 U/g(以底物计),底物浓度2.5%,此时ACE抑制率为70.1%,水解度为17.5%,与模型预测值基本相符。

Pinus koraiensis Sieb.et Zucc protein isolate was hydrolyzed with alcalase to prepare antihypertensive peptides. Operating parameters such as pH, temperature, substrate concentration and enzyme dosage were optimized using response surface analysis based on degree of hydrolysis and ACE inhibitory rate. The optimum hydrolysis conditions for ACE inhibitory rate （70.1%） and degree of hydrolysis （17.5%） were found to be hydrolysis at 55℃, pH 8.9 for 3 h with an enzyme/substrate ratio of 7 500 U/g and substrate concentration of 2.5%, with the model prediction.