蜂胶中黄酮的提取及其自由基清除活性研究

Study on extraction of flavonoids from propolis and the activity of scavenging free radicals

以蜂胶为原料,在单因素实验的基础上,通过正交实验优化超声波提取蜂胶黄酮工艺,并对其抗氧化性进行评价。结果表明,影响黄酮提取的因素主次顺序为:料液比>乙醇浓度>超声时间>提取温度,最佳提取工艺组合为A2B2C2D2,即料液比为1∶30、乙醇浓度为80%、超声时间为20min、提取温度为50℃,在此工艺条件下蜂胶中黄酮的提取率为9.60%。蜂胶中的黄酮对羟基自由基和超氧阴离子自由基具有较好的抑制作用且具有明显的量效关系,即随着提取物浓度的增加,对自由基的清除活性逐渐增强;当提取物添加量为0.15 mg/m L时具有较好的自由基清除作用,其抗氧化活性优于天然抗氧化剂VC。

Taking propolis as raw material, based on single factor experiments, orthogonal test was used to optimize the ultrasonic - assisted extraction of flavonoids, and antioxidant activity of the total flavonoids was studied. The results showed that the order of factors affecting the extraction was ratio of solid to liquid 〉 ethanol concentration 〉 extraction time 〉 extraction temperature ; the optimum extraction technology was A2B2CED2, namely ratio of solid to liquid 1： 30, ethanol concentration 80%, extraction time 20 min, ex- traction temperature 50 ℃, under the condition the extraction rate of flavonoids in propolis was 9.60%. Propolis flavonoids had obvious inhibition effect on hydroxy radical and superoxide anion free radical, with obvious dose effect relationship, namely the ability of eliminating free radical strengthened along with the increasing of ethanolic extract density. Flavonoids of propolis with addition amount of 0.15 mg/mL had good antioxidant activity in scavenging free radicals, which was better than natural antioxidant Vc.