玫瑰茄花色苷纯化工艺优化研究

Research on purification of roselle anthocyanins

对玫瑰茄花色苷纯化工艺进行优化研究,通过对X-5、DA-201、HPD-100、D101、AB-8五种大孔树脂进行筛选,得出最适用于玫瑰茄花色苷纯化的树脂为HPD-100大孔树脂。通过单因素和正交实验对HPD-100大孔树脂纯化条件进行优化,得出最佳吸附工艺条件是:样液浓度为1.5 mg/m L,上样流速为2 m L/min,p H值为3.0,吸附率为91.988%;最佳解吸工艺条件是:洗脱液浓度为70%,洗脱流速为1.75 m L/min,洗脱液用量60 m L,解吸率为92.144%,纯化后的玫瑰茄红色素色价为38.5。通过纯化前后玫瑰茄红色素高效液相色谱图的比较可知:玫瑰茄红色素主要含有5种成分,纯化后的色素矢车菊花色苷的含量明显升高。

The purification of roselle anthocyanins was studied. The most suitable resin for roseUe antho- cyanin purification was HPD - 100 macroporous resin, which was obtained by screening five kinds of macroporous resins： X - 5, DA - 201, HPD - 100, D101 and AB - 8. The purification conditions for HPD -100 macroporous resin were optimized by single factor and orthogonal experiments, which were： the sample＇s concentration 1.5 mg/mL, flow rate 2 mL/min, pH 3. The adsorption rate can reach 91. 988%. The optimal desorption process conditions were eluent concentration 70%, elution velocity 1.75 mL/min, dosage of eluent 60 mL. The desorption rate can reach 92. 144%. After the purification, the color value of roselle anthocyanins was 38.5. Compared before and after the purification, the chro- matograms by HPLC showed that roselle red pigment mainly contained five components. The cornflower anthocyanin content in the pigment increased significantly after being purified.