姜黄素抗小鼠原发性肝泡球蚴病的实验研究

Effect of curcumin on primary hepatic alveolar echinococcosis in mice

目的观察姜黄素对小鼠原发性肝泡球蚴病的影响。方法昆明小鼠肝脏感染泡球蚴60d后随机分为3组,即模型对照组,姜黄素溶剂组,姜黄素组(200mg/kg·d),另设空白对照组。用相应药物治疗60d,检测各组小鼠泡球蚴囊湿重,计算囊湿重抑制率,并进行HE染色观察病理组织学改变。结果药物治疗60d后姜黄素组、模型对照组及姜黄素溶剂组小鼠囊湿重分别为(0.2638±0.092)g、(0.4890±0.084)g和(0.4558±0.098)g,姜黄素组与模型对照组对比差异有统计学意义(P<0.05),姜黄素组囊抑制率为46.05%;姜黄素溶剂组与模型对照组的囊湿重比较差异无统计学意义(P>0.05)。肝泡球蚴组织发生Ⅰ(变性)、Ⅱ(坏死)级病理变化的小鼠占比分别为姜黄素组62.50%,模型对照组为5.90%,姜黄素溶剂组为0.00%,姜黄素组与模型对照组对比差异有统计学意义(P<0.05);姜黄素溶剂组与模型对照组比较差异无统计学意义(P>0.05)。结论姜黄素能起到一定的抗小鼠原发性肝泡球蚴病的作用。

Objective The effect of curcumin on primary hepatic alveolar echinococcosis in mice was observed in this experimental research. Methods Kunming mice infected with Echinococcus multilocularis for 60 days were randomly divided into 3groups：a model control group,agroup given curcumin in solution,and a group given curcumin（200mg/kg· d）.Healthy mice served as the blank control group.After mice were given the corresponding treatment for 60 days,the wet weight of E.multilocularis cysts and the rate of inhibition of E.multilocularis cysts were determined.The cysts were removed and HE stained was used to observe resulting pathological changes. Results After 60 days of treatment,the wet weight of E.multilocularis cysts was 0.2638±0.092 g in the group given curcumin,0.4890±0.084 g in the model control group,and 0.4558±0.098 g in the group given curcumin in solution.The wet weight of cysts differed significantly between the group given curcumin and the model control group as well as between the group given curcumin and the group given curcumin in solution（P〈0.05）.The rate of inhibition of E.multilocularis cysts was 46.05% in the group given curcumin,but there were no statistical differences（P〉0.05）in the wet weight of E.multilocularis cysts for the group given curcumin in solution and the model control group.Pathological changes indicated that the rate of pathological changes（grade I,degeneration;grade II,necrosis）in mice with liver tissues infected with E.multilocularis was62.50%in the group given curcumin,5.90%in the model control group,and in 0.00%in the group given curcumin in solution.There were statistically significant differences（P〈0.05）in the rate of pathological changes between the group given curcumin and the model control group as well as between the group given curcumin and the group given curcumin in solution.However,there were no statistically significant differences（P〉0.05）in the rate of pathological changes between the group given curcumin in solution and the model control group. Conclusion Experimental results indicated that curcumin may play a certain role in producing resistance to primary hepatic alveolar echinococcosis in mice.