HPLC测定肾益康胶囊中大黄酚和大黄素的含量

Determination of Chrysophanol and Emodin in Shenyikang Capsules by HPLC

目的：建立测定肾益康胶囊中大黄酚和大黄素含量的高效液相色谱法。方法：色谱柱为SB-C18柱（150mm×4.6mm,5μm）,流动相为甲醇-0.1%磷酸溶液（85∶15）,流速1.0m L/min,检测波长254nm,柱温30℃。结果：大黄酚在1.996～19.96μg（r=0.9995）范围内与峰面积线性关系良好,平均回收率为98.20%,RSD为1.63%（n=6）。大黄素在4.18～41.8μg（r=0.9996）范围内与峰面积线性关系良好,平均回收率为98.25%,RSD为1.78%（n=6）。结论：该方法简便、快速、准确,可较好控制该制剂的质量。

Objective ：To establish the HPLC method for determining the content of ehrysophanol and emodin in Shenyikang Capsules. Methods：The HPLC method was used with the S B - C18 column （150mm×4.6mm,5μm）, The mobile phase consisted of methanol -0.1% phosphoric acid solution（85：15 ）, The flow rate was 1.0mL/min, the detection wavelength was 254 nm, and the column temperature was 30℃. Results：The calibration curve of chrysophanol was linear in the range of 1.996～ 19.96μg（r=0.9995） .The average recovery rate was 98.20%,RSD=1.63%（n=6）. The calibration curve of emodin was linear in the range of 4.18～4.18μg （r=0.9996）.The average recovery rate was 98.25%, RSD =1.78% （n=6）. Conclusion：The method is simple, rapid and accurate, And it can control the quality of the preparation, effectively.