丙型肝炎病毒NS3-5B转基因小鼠模型的构建

Generation of the Transgenic Mice Model Expressing HCV NS3-5B

目的:构建表达丙型肝炎病毒(HCV)NS3-5B蛋白的转基因小鼠模型。方法:显微注射线性化p IRES2-EGFP-NS3-5B载体到小鼠受精卵,制备并传代筛选HCV NS3-5B转基因小鼠;通过血清谷丙转氨酶(ALT)、谷草转氨酶(AST)检测和肝脏HE染色,对6周龄转基因小鼠进行肝功能评价。结果:PCR、RT-PCR和Western印迹结果表明HCV NS3-5B转基因小鼠构建成功;6周龄部分转基因小鼠血清ALT和AST值升高,但差别无统计学意义,肝组织形态无改变。结论:构建了HCV NS3-5B转基因小鼠模型,为进一步在体研究HCV复制复合体建立了平台。

Objective： To construct the transgenie mice model expressing HCV NS3-5B protein. Methods： HCV NS3-5B transgenic mice were generated by microinjection of the linearized vector of pIRES2-EGFP-NS3-5B into mice fertilized eggs. Subsequently, homozygous transgenic mice with genetic stability were selected by breeding. Liv- er function of 6 weeks old transgenic mice were evaluated via testing ALT and AST in serum and HE staining of liver. Results： NS3-5B transgenic mice were identified by PCR, RT-PCR and Western blotting. Compared with the same age of wild type C57BL/6, the change of ALT and AST of the transgenic mice were no statistical differ- ence and the shape of liver tissue was unchanged. Conclusion： The transgenic mouse model expressing HCV NS3- 5B was generated successfully. It provides a research platform for HCV replication complex in vivo.