MALDI-TOF质谱检测单条植物线虫

Rapid Detection of Single Plant Nematode via MALDI-TOF Mass Spcectrometry

目的:研发一种快速、灵敏检测植物线虫的方法。方法:挑取单条线虫到裂解液中,用挑针压破线虫,以便提高蛋白质提取效率,利用MALDI-TOF质谱技术分析线虫的蛋白质指纹图谱,作为结果判断依据。结果:用该方法分析单条短体线虫的雌虫和二龄幼虫,雌虫的质谱峰值分别为m/z 2790、2970、3326、4373、4872、5654、7000、9579和19161,二龄幼虫的质谱峰值分别为m/z 2185、2790、3036、3326、4063、4373、4929、5380、6999和11156,二者相同的峰值分别为m/z 2790、4373和7000,其余峰值分别为各自的特征峰。结论:MALDI-TOF质谱能够区分同种线虫的不同虫态,检测灵敏度高,可用于单条植物线虫的检测。

Objective： To establish a rapid and sensitive method for detecting the plant nematode. Methods： A single nematode was picked into lysis liquid and crushed to improve the protein extraction efficiency. MALDI- TOF MS was used to analysis the protein spectra of single nematode. Results： The methode was used to analysis the single adult Pratylenchus spp and their second juvenile（J2） based on mass spectrometric profilling. Peaks at m/ z 2970, 3326, 4872, 5654, 9579, 19161 were only present in the adult, while m/z 2185, 3036, 3326, 4063, 4929, 5380, 11156 were unique to the J2. The common peaks to both the phases were at m/z 2790, 4373, 7000. Con- clusion： MALDI-TOF MS can be used to differentiate between the different stage of the nematode and the use of this method for efficient, sensitive and stable detection of a single nematode is feasible.