EAE大鼠血清中TNF-α、IFN-γ的检测及意义

The detection and significance of TNF-α、IFN-γ in EAE Rats' serum

目的探讨干扰素-γ(IFN-γ)和肿瘤坏死因子-α(TNF-α)对大鼠实验性自身免疫性脑脊髓炎(experimental autoimmune encephalomyelitis,EAE)的作用。方法 Wistar大鼠通过局部注射豚鼠全脊髓匀浆和完全弗氏佐剂制成的抗原乳剂诱导EAE模型,应用双抗夹心ELISA、放射免疫分析法分别检测实验大鼠血清IFN-γ和TNF-α的表达,并观察两种细胞因子的改变与EAE症状之间的关系。结果诱导EAE后,伴随着大鼠EAE症状的出现和进行性加重,其血清中IFN-γ和TNF-α表达较正常组分别升高了173.86%和166%。结论 IFN-γ和TNF-α在EAE的发生和发展密切相关,这两种细胞因子在EAE发病机制中的重要作用为探索新的有效治疗多发性硬化症(multiple sclerosis,MS)的手段提供了重要理论依据。

[ Objective ] To investigate the role of interferon-γ （IFN- γ） and tumor necrosis factor- α （TNF- α ） in experimental allergic encephalomyelitis（EAE）. [ Methods ] The EAE models were made in female Wistar rats by having been immuned with spinal cord extracts together with complete Freund＇s adjuvant. The serum IFN- γ was examined by ELISA and TNF- α was examined by radioimmunity assay. The relationship between IFN- γ and TNF- α and symptoms of EAE were also investigated. [ Results ] After EAE was induced, the levels of serum IFN- γ and TNF- α were increased by 173.86% and by 166% respectively with the development of symptoms of EAE. [ Conclusions ] The serum IFN- γ and TNF- α are closely related to the occurrence and development of EAE. The important roles of IFN- α and TNF- α in EAE provide an important theoretical basis for exploring the new multiple sclerosis （MS） treatment.