川藏黑猪和杜长大猪血清中瘦素的发育性变化研究

The Research on Developmental Changes of Leptin in Serum of Pigs

为了解川藏黑猪和杜长大猪血清中瘦素的发育性变化,采用酶联免疫吸附试验方法检测不同体重梯度(90 kg、110 kg和130 kg)的川藏黑猪和杜长大猪血清中瘦素的含量,并分析了其与肌内脂肪含量的关系。结果表明,川藏黑猪在90 kg、110 kg和130 kg体重时血清中瘦素含量分别为1 106.39 ng/L、1 168.04 ng/L和1 146.10 ng/L,肌内脂肪含量分别为3.72%、4.41%和4.96%;杜长大猪在90 kg、110 kg和130 kg体重时血清中瘦素含量分别为1 032.52 ng/L、1 002.24 ng/L和1 006.96 ng/L,肌内脂肪含量分别为2.00%、2.50%和2.61%。90 kg、110 kg和130 kg体重时川藏黑猪肌内脂肪含量显著或极显著高于杜长大猪。血清中瘦素含量,在品种内不同体重梯度间差异均不显著(P>0.05);不同猪种间,90 kg梯度两猪种间差异不显著,110 kg、130 kg梯度川藏黑猪均极显著高于杜长大猪(P<0.01)。相关性分析显示,瘦素含量与肌内脂肪含量成极显著正相关(P<0.01),表明瘦素可能通过血液对猪肌内脂肪的沉积进行调控。

In this article,Chuanzang Black Swine Hybrid and DLY pigs of 3 weights（90 kg/110 kg/130 kg） were selected to investigate the developmental changes of leptin content in serum by enzyme-linked immunosorbent assay （ELISA）,their relationship with intramuscular fat content were also analyzed.The results showed that the leptin content of Chuanzang Black Swine Hybrid of 90 kg, 110 kg,130 kg bodyweight were 1 106.39 ng/L, 1 168.04 ng/L and 1 146.10 ng/L respectively,and IMF content respectively were 3.72%,4.41% and 4.96%;leptin content of DLY pigs were 1 032.52 ng/L,1 002.24 ng/L and 1 006.96 ng/L respectively,while IMF content respectively were 2.00%,2.50% and 2.61%.IMF content of Chuanzang Black Swine Hybrid of different weights were both sig- nificant higher than DLY pigs.There was no significant difference in leptin content of different weight of the same breed（P〉0.05）.The leptin content of Chuanzang Black Swine Hybrid were both significant higher than DLY pigs of 110 kg and 130 kg（P〈0.01）,but no significant difference at 90 kg（P〉0.05）.Relevance analysis showed that leptin contenthad a significant positive correlation （P〈0.01） with the intramuscular fat content,which prompt that leptin may regulatethe deposition of intramuscular fat through blood.