新疆石河子地区汉族人群HLA-DPB1基因多态性与肺结核易感的相关性研究

HLA-DPB1 gene polymorphism and susceptibility to tuberculosis in Xinjiang Shihezi Han population: a case-control study

目的:探讨研究新疆石河子地区的汉族肺结核患者与汉族健康对照者的人类白细胞抗原(HLA)-DPB1等位基因的多态性及其与肺结核易感的相关性。方法:采用DNA水平基于测序的分型方法(Sequence-based typing,SBT),通过病例-对照研究93例新疆石河子地区汉族肺结核患者与96例同一地区的汉族随机健康对照者的HLA-DPB1位点的多态性,通过统计分析研究结果,筛选出易感和保护性基因。结果:在健康对照组中,共筛选出16个等位基因,其中HLA-DPB1*0501(28.1%)和HLA-DPB1*0201(27.6%)的基因频率显著高于其他位点,分别位居第一第二;病例组与对照组研究发现,HLADPB1*0201在对照组中的频率显著高于病例组(P<0.05)。而HLA-DPB1*0501在病例组的频率显著高于对照组(P<0.05)。结论:新疆石河子地区汉族人群HLA-DPB1基因频率与中国其他地区北方汉族人群大体一致;同时本研究提示HLADPB1*0501可能为新疆石河子地区汉族肺结核的易感性基因,而HLA-DPB1*0201则可能为其保护性基因。

Objective：In this study,we examined the HLA-DPB1 alleles in patients with tuberculosis,and health individuals attempt to investigate the association between the polymorphism of HLA-DPB1 gene and pulmonary tuberculosis in Han population in Shihezi area in Xinjiang uygur autonomous region of China.Methods：High-resolution typing of DPB1 was performed by the sequence-based typing （ SBT） method using the SBT-HLA-DPB1 generic DNA typing kit.Results：In the controls ,17 HLA-DPB1 alleles were ob-served,the HLA DPB1＊0501 （28.1%） and HLA DPB1＊0201（27.6%） frequency was significantly higher than other sites ,the first and second respectively.The frequency of HLA-DPB1＊ 0201 was observed significantly increased in patient group compared with control group （ P〈0.05 ）;the frequency of HLA-DPB1＊ 0501 was observed significantly decreased in patient group compared with control group （ P〈0.05 ）.Conclusion： The gene frequencies of HLA-DPB1 in Xinjiang Shihezi Han population are roughly in consistence with other northern Chinese Han population;the HLA-DPB1＊0201 may be the protective factor to pulmonary tuberculosis , and HLA-DPB1＊0501 may be susceptible to pulmonary tuberculosis in Han population from the Xinjiang uygur autonomous region of China.