铜仁地区肠道病毒EV71基因分型与体液免疫水平关系的研究

Research enterovirus EV71 genotyping Tongren and humoral immune function relationships

目的:探究分析铜仁地区肠道病毒EV71基因分型与体液免疫功能的关系,为临床进一步认识肠道病毒EV71提供参考依据。方法:选择2014年2月至2014年8月铜仁地区来我院诊治的肠道病毒EV71感染的手足口病患儿,根据感染主要病毒的基因类型分为三组,即A组,主要有A型肠道病毒EV71感染,20例患儿;B组,主要有B型肠道病毒EV71感染,20例患儿;C组,主要有C型肠道病毒EV71感染,20例患儿。通过检测三组患者的免疫球蛋白及TNF-ɑ、IL-6以及IL-10的水平,比较不同基因型的肠道病毒EV71对手足口病患儿体液免疫功能的影响。结果:经过检测,A组患者的TNF-ɑ、IL-6以及IL-10水平分别为(285.60±30.50)pg/L、(60.50±5.60)pg/L、(25.50±4.50)pg/L,均较B组、C组患者的三项指标的水平高,具有显著性差异(P<0.05),C组患者的TNF-ɑ、IL-6以及IL-10水平均较B组患者的三项指标的水平高,具有明显差异性(P<0.05);经过检测,三组患者的Ig A、Ig G水平两两比较均存在显著性差异(P<0.05),A组患者的Ig A、Ig G水平最高,C组次之,B组患者水平最低;三组患者的Ig M的水平两两比较无显著差异性(P>0.05)。结论:肠道病毒EV71的不同基因型均能引起严重的手足口病,均能显著提高血清炎症因子的水平以及免疫球蛋白的水平,但不同基因型的致病力度不同,存在一定的差异性,但具体机制需要进一步探究。

Objective：To explore the relationship between Tongren analysis EV 71 intestinal virus genotyping and humoral immune function ,for further clinical understanding of enterovirus EV 71 provide references.Methods：February to August 2014 Tongren to our hospital for treatment of intestinal virus EV 71 HFMD infected children ,according to the genetic type of virus infection is mainly divided into three groups ,namely group A,there were A type of intestinal virus EV 71 infection,20 patients;group B,there were B-type intestinal virus EV71 infection,20 patients;group C,there were C-type intestinal virus EV71 infection,20 cases of children.Three groups of patients by detecting immunoglobulin levels and TNF-ɑ, IL-6 and IL-10 levels, and to compare different genotypes affect intestinal virus EV71 HFMD children humoral immune function.Results：After testing,A group of patients with TNF-ɑ,IL-6 and IL-10 levels were （285.60 ±30.50） pg/L,（60.50 ±5.60） pg/L,（25.50 ±4.50） pg/L,than those in group B,high levels of three indicators of group C patients ,a significant difference （ P〈0.05 ） ,group C patients with TNF-ɑ,IL-6 and IL-10 levels in three groups of patients than B high level indicators ,with a significant difference （ P〈0.05 ）;tested three groups of patients with IgA ,IgG level pairwise comparisons showed that there were significant differences （P〈0.05）,the highest a group of patients with IgA,IgG level,group C, followed by the lowest level of group B patients;levels of the three groups of patients with IgM pairwise no significant difference （ P〉0.05 ） .Conclusion：Different genotypes of enterovirus EV 71 can cause serious foot and mouth disease ,and could significantly improve the level of serum immunoglobulin levels of inflammatory cytokines ,but the intensity of different genotypes of different pathogenic ,there are some differences ,but the specific mechanisms need to be further explored.