姜黄素抑制小鼠生殖器官氧化产物生成的研究

Inhibit effect of curcumin on lipid peroxidation product in the reproductive organs of mice

目的:探讨姜黄素对氧化应激小鼠子宫、卵巢组织脂质过氧化水平的影响。方法:昆明种雌性成年小鼠40只,随机分成正常对照组,0 mg/kg Cur组,100 mg/kg Cur组,200 mg/kg Cur组,连续用药21天,实验结束后测定小鼠子宫、卵巢组织以及血清中丙二醛(MDA)、活性氧(ROS)的含量。结果:100 mg/kg Cur组,200 mg/kg Cur组血清、子宫以及卵巢氧化应激产物ROS、MDA与砷暴露组相比均有降低,具有明显统计学差异(P<0.05)。结论 :姜黄素可降低小鼠子宫、卵巢组织以及血清中氧化产物MDA、ROS的含量,对小鼠卵巢氧化应激引起的生殖器官损伤有保护作用。

Objectives：To observe the different effect of curcumin on the markers of oxidative stress to explore the pathogenesis to offer more experimental evidence for the clinical use of cur cumin on the oxidative stress related ovari-an diseases.Methods：40 Kunming mice with two regular estrus cycles,without coitus and body -matured female werenbsp;selected as research objects and were randomly divided into four groups,including control group,0mg/kg Cur group, 100mg/kg Cur group,200mg/kg Cur group.All rats were treated for 21days and examined by ELISA for the concentration of Reactive Oxygen Species （ROS）,Malondialdehyde （MDA）in serum,uterus and ovaries.Results：Compared with the 0mg/kg Cur,the concentrations of ROS and MDA in the serum,uterus and ovaries of 100mg/kg Cur group and 200mg/kg Cur group were decreased,the difference was significant （P 〈0.05）.Conclusions：Curcumin can reduce the concentration of ROS,MDA.We conclude that curcumin can scavenge free radicals in mouse reproductive system.From this way,curcumin has good antioxidant capacity in mouse reproductive sysyterm.